ETS1 associated protein II Antibody

Western Blot: ETS1 associated protein II Antibody [NBP1-46178] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-TDP2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. TDP2 was also immunoprecipitated by another rabbit anti-TDP2 antibody, which recognizes a downstream epitope.

Immunohistochemistry: ETS1 associated protein II Antibody [NBP1-46178] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TDP2 used at a dilution of 1:200 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ETS1 associated protein II Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 50 and 100 of human Tyrosyl DNA Phosphodiesterase-2 using the numbering given in entry NP_057698.2 (GeneID 51567).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TDP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ETS1 associated protein II Antibody

• AD022
• dJ30M3.3
• EAP2
• EAPII
• EC 3.1.4.-
• ETS1-associated protein 2
• ETS1-associated protein II
• MGC111021,5'-Tyr-DNA phosphodiesterase
• MGC9099,5'-tyrosyl-DNA phosphodiesterase
• TRAF and TNF receptor associated protein
• TTRAPTRAF and TNF receptor-associated protein
• Tyr-DNA phosphodiesterase 2
• tyrosyl-DNA phosphodiesterase 2

Background

Ets proteins constitute a family of conserved sequence-specific DNA-binding proteins and function as transcription factors with roles in differentiation, lymphoid cell development, invasiveness and angiogenesis. A novel ETS1 interacting protein designated as ETS1-associated protein II (EAPII) interacts with ETS1 and other Ets proteins (ETS2 and FLI1) and is a novel ETS1 modulator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ETS1 associated protein II Antibody (NBP1-46178)(1)

Publication using NBP1-46178

• Virgen-Slane R, Rozovics JM, Fitzgerald KD et al. An RNA virus hijacks an incognito function of a DNA repair enzyme. Proc Natl Acad Sci U S A 2012-09-01 [PMID: 22908287]

Bioinformatics

• Gene Symbol: TDP2
• Entrez:
  • 51567(Human)
• Uniprot:
  • O95551(Human)