ESRP2 Antibody (22C5.H7.A6) - BSA Free

Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972] - Anti-ESRP2 by Detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2.

• Reactivity: Mu
• Applications: WB, ELISA
• Clone: 22C5.H7.A6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ESRP2 Antibody (22C5.H7.A6) - BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: ESRP2 Antibody (22C5.H7.A6) is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein. (Uniprot: Q8K0G8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESRP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100000
  • Western Blot 1:1000
• Application Notes: This product has been tested for use western blotting and ELSIA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with ESRP2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with ESRP2 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for ESRP2 Antibody (22C5.H7.A6) - BSA Free

• epithelial splicing regulatory protein 2
• FLJ21918
• FLJ22248
• RBM35BRNA-binding protein 35B
• RNA binding motif protein 35A
• RNA binding motif protein 35B
• RNA-binding motif protein 35B

Background

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ESRP2 Antibody (NBP1-77972)(1)

Publication using NBP1-77972

• Warzecha CC, Jiang P, Amirikian K et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 2010-10-01 [PMID: 20711167]

Bioinformatics

• Gene Symbol: ESRP2
• Uniprot:
  • Q8K0G8(Mouse)