ESRP2 Antibody (22C5.H7.A6)

Images

 
Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972] - Anti-ESRP2 by Detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
22C5.H7.A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ESRP2 Antibody (22C5.H7.A6) Summary

Immunogen
Full length recombinant mouse Esrp-2 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% identity with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESRP2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100000
Application Notes
This protein-A purified antibody has been tested for use western blotting and shows specific reactivity by with ESRP2 transfected cell lysates.. Expect a band approximately 77.4 kDa in size corresponding - Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Publications
Read Publication using NBP1-77972.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for ESRP2 Antibody (22C5.H7.A6)

  • epithelial splicing regulatory protein 2
  • FLJ21918
  • FLJ22248
  • RBM35BRNA-binding protein 35B
  • RNA binding motif protein 35A
  • RNA binding motif protein 35B
  • RNA-binding motif protein 35B

Background

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ESRP2 Antibody (NBP1-77972)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ESRP2
Uniprot