ELISA: Mouse Erythropoietin R ELISA Kit (Colorimetric) [NBP2-67948] - Samples were spiked with high concentrations of Mouse Erythropoietin R and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Mouse Erythropoietin R ELISA Kit (Colorimetric) [NBP2-67948] - Standard Curve Reference
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent
Alternate Names for Mouse Erythropoietin R ELISA Kit (Colorimetric)
EpoR
EPO-R
Erythropoietin R
erythropoietin receptor
MGC138358
Background
The human erythropoietin receptor (EpoR) is located on stem and mature cells of the hematopoietic system. When activated by its native ligand, erythropoietin (Epo), EpoR mediates proliferation and maturation of the erythroid precursor cells leading to expansion of red cell populations. Human recombinant Epo is currently used for treatment of patients undergoing erythrocyte depletion due to dialysis for kidney failure.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Erythropoietin R ELISA Kit (NBP2-67948). (Showing 1 - 1 of 1 FAQ).
What is the difference between the Quantikine and Duoset ELISA kits?
Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.