Recombinant Human Erythropoietin/EPO Protein

Product Discontinued

Recombinant Human Erythropoietin/EPO Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-193 of Human Erythropoietin
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAPPRLIC DSRVLERYLL EAKEAENITT GCAEHCSLNE NITVPDTKVN FYAWKRMEVG QQAVEVWQGL ALLSEAVLRG QALLVNSSQP WEPLQLHVDK AVSGLRSLTT LLRALRAQKE AISPPDAASA APLRTITADT FRKLFRVYSN FLRGKLKLYT GEACRTGDR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EPO
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human Erythropoietin/EPO Protein

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin, also known as EPO, is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. Overexpression of EPO is associated with several pathophysiological conditions, such as polycythemias vera, which is caused by the EPO -independent growth of erythrocytic progenitors from abnormal stem cells. Recombinant human EPO protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EPO