Erythropoietin/EPO Antibody [Unconjugated] Summary
Immunogen |
Chinese hamster ovary cell line (CHO)-derived recombinant human Erythropoietin/EPO |
Specificity |
Detects human Erythropoietin/EPO in direct ELISAs and Western blots.
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Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
EPO |
Purity Statement |
Protein A or G purified |
Endotoxin Note |
<0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 5-15 ug/mL
- Neutralization <3 ug/mL
- Western Blot 1 ug/mL
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Reviewed Applications |
Read 1 Review rated 4 using AB-286-NA in the following applications:
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Publications |
Read Publications using AB-286-NA in the following applications:
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 1 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Erythropoietin/EPO Antibody [Unconjugated]
Background
Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of EPO is required for biological activities in vivo. Mature human EPO shares 75%‑84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha . EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for Erythropoietin/EPO Antibody (AB-286-NA) (0)
Secondary Antibodies
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Isotype Controls
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