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Erythropoietin/EPO Antibody [Unconjugated]

 (3 Publications)
 (1 Review)
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Erythropoietin/EPO was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Erythropoietin/EPO was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Erythropoietin/EPO Polyclonal Antibody (Catalog # AB-286-NA) at 15 µg/mL for 1 hour at room ...read more
Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in theTF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Erythropoietin/EPO Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line (CHO)-derived recombinant human Erythropoietin/EPO
Specificity
Detects human Erythropoietin/EPO in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPO
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization <3 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AB-286-NA in the following applications:
Publications
Read Publications using
AB-286-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin/EPO Antibody [Unconjugated]

  • ECYT5
  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of EPO is required for biological activities in vivo. Mature human EPO shares 75%‑84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells. Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha . EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Erythropoietin/EPO
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AB-286-NA
Erythropoietin/EPO Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N

Publications for Erythropoietin/EPO Antibody (AB-286-NA)(3)

We have publications tested in 2 confirmed species: Human, Transgenic Hamster.

We have publications tested in 3 applications: ELISA Detection, ICC, Western Blot.


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Review for Erythropoietin/EPO Antibody (AB-286-NA) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AB-286-NA:
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(1)
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(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous -
 Western Blot Other 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 19127370
SpeciesOther

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Recent Reviews

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 Anonymous -
01/05/2015
Application: Western Blot
Species: Other
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Bioinformatics

Gene Symbol EPO