Human Erythropoietin ELISA Kit (Colorimetric)

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ELISA: Erythropoietin ELISA Kit [NBP2-31082] - In an experiment involving 9 EDTA- and 15 heparin plasma samples, as well as 15 serum samples, NBP2-31082 and competitor ELISA Kit show a correlation factor of R = 0.973.
Sandwich ELISA: Human Erythropoietin ELISA Kit (Colorimetric) [NBP2-31082] - Representative standard curve for human Erythropoietin ELISA. Human Erythropoietin was diluted in serial 2-foldsteps in Sample Diluent. Do not ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
1.6 - 100 mIU/mL
Sensitivity
0.17 mIU/mL

Order Details

Human Erythropoietin ELISA Kit (Colorimetric) Summary

Standard Curve Range
1.6 - 100 mIU/mL
Sensitivity
0.17 mIU/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.5%
Intra-Assay
7.8%
Spike Recovery
105.2
Sample Volume
50 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
EPO

Applications/Dilutions

Application Notes
The human Erythropoietin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Erythropoietin.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Control high, lyophilized
  2. Control low, lyophilized
  3. Human Erythropoietin Standard lyophilized, 200 mIU/mL upon reconstitution
  4. Adhesive Films
  5. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Erythropoietin
  6. Biotin-Conjugate anti-human Erythropoietin monoclonal antibody
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. Sample Diluent
  9. Stop Solution (1M Phosphoric acid)
  10. Streptavidin-HRP
  11. Substrate Solution (tetramethyl-benzidine)
  12. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human Erythropoietin ELISA Kit (Colorimetric)

  • EP
  • EPO
  • epoetin
  • Erythropoietin
  • MGC138142
  • MVCD2

Background

Erythropoietin (EPO) is a hormone produced by the kidney that promotes the formation of red blood cells in the bone marrow. EPO is a glycoprotein with a molecular weight of 34 kDa. The kidney cells that primarily make EPO are specialized and sensitive to low oxygen levels in the blood. These cells release EPO when the oxygen level is low in the kidney. EPO binds the Epo receptor (Epo R) on erythroid progenitors in bone marrow eliciting proliferation, maturation, and differentiation of red blood cells thereby increase the oxygen-carrying capacity of the blood. EPO is the prime physiological regulator of red blood cell production. The measurement of EPO in the blood is useful in the study of bone marrow disorders and kidney disease. Elevated levels of EPO can be seen in polycythemia, a disorder in which there is an excess of red blood cells. Lower than normal levels of EPO are seen in chronic renal failure. EPO plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process. Measurement of serum immunoreactive erythropoietin has shown that overproduction of EPO can be an adaptive response to conditions producing tissue hypoxia, such as smoking chronic obstructive pulmonary disease, renal hypoxia or cyanotic heart disease. Elevated serum levels have further been found in patients suffering from various neoplastic diseases, such as renal carcinomas and benign renal tumors, liver carcinomas and hepatomas, cerebellar hemangioblastomas and other neoplastic disorders. Serum levels of EPO lower than normal are found in various forms of anemias. In case EPO is not primarily involved in the cause of the anemia, elevated levels of EPO are found in serum of the patients suffering from diseases like aplastic anemias, iron deficiency, megaloblastic anemias, thalassemias and myelodysplastic syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Erythropoietin ELISA Kit (NBP2-31082). (Showing 1 - 2 of 2 FAQ).

  1. Our customer wants to know the origin of cell for erythropoietin in NBP2-31082. Could you let me know?
    • Our EPO ELISA kit with catalogue number NBP2-31082 is designed to be used for the quantitative detection of human Erythropoietin. Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), have all been tested with this assay. Our lab informed me that to test the suitability of this kit for use with cell culture samples, we spiked cell culture medium (RPMI medium) with 10% FCS. The erythropoietin was obtained from recombinant EPO expressed in CHO cells.
  2. Could you tell me how many plates are supplied in the NBP2-31082?
    • NBP2-31082 contains one 96 well microtitre plate.

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Blogs on Erythropoietin.

HIF-2 alpha Antibody Throws New Light on EPO Dilemma
The HIF family are heterodimeric, oxygen-sensitive transcription factors comprising an alpha and beta subunit which are normally dissociated in normoxic conditions. Our antibody catalog contains products targeting all the Hypoxia Inducible Factor isof...  Read full blog post.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

Hypoxia-Inducible Factors and Homeostasis
Hypoxia-Inducible Factor-1 (HIF-1) is a highly conserved heterodimeric transcription factor. Novus' antibody catalogue contains an extensive range of both HIF-1 alpha and HIF-1 beta, useful for hypoxia, angiogenesis, cancer and many other areas of res...  Read full blog post.

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Bioinformatics

Gene Symbol EPO
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