Recombinant Human ERR beta/NR3B2 GST (N-Term) Protein

• Reactivity: Hu
• Applications: WB, ELISA, MA

Recombinant Human ERR beta/NR3B2 GST (N-Term) Protein Summary

• Description: Human ESRRB (AAI48478.1, 1 a.a.-500 a.a.) recombinant protein with GST tag at N-terminal.
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MSSDDRHLGSSCGSFIKTEPSSPSSGIDALSHHSPSGSSDASGGFGLALGTHANGLDSPPMFAGAGLGGTPCRKSYEDCASGIMEDSAIKCEYMLNAIPKRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYSCPATNECEITKRRRKSCQACRFMKCLKVGMLKEGVRLDRVRGGRQKYKRRLDSESSPYLSLQISPPAKKPLTKIVSYLLVAEPDKLYAMPPPGMPEGDIKALTTLCDLADRELVVIIGWAKHIPGFSSLSLGDQMSLLQSAWMEILILGIVYRSLPYDDKLVYAEDYIMDEEHSRLAGLLELYRAILQLVRRYKKLKVEKEEFVTLKALALANSDSMYIEDLEAVQKLQDLLHEALQDYELSQRHEEPWRTGKLLLTLPLLRQTAAKAVQHFYSVKLQGKVPMHKLFLEMLEAKAWARADSLQEWRPLEQVPSPLHRATKRQHVHFLTPLPPPPSVAWVGTAQAGYHLEVFLPQRAGWPRAA

• Preparation Method: in vitro wheat germ expression system
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ESRRB
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Protein Array
  • Western Blot
• Theoretical MW: 81.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Recombinant Human ERR beta/NR3B2 GST (N-Term) Protein

• ERR beta
• ERR2
• ESRL2
• ESRRB
• estrogen-related receptor beta
• NR3B2

Background

Estrogen-related receptor beta (ERR beta) a NR3 Steroid Receptor, has been shown to affect early placental development. ERR beta also can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). ERR beta binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). ERR beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. ERR beta expression has been documented in mice in the extraembryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ESRRB