ERp72 Antibody

Western Blot: ERp72 Antibody [NBP2-16371] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ERp72 antibody diluted at 1:500 and competitor's antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ERp72 Antibody [NBP2-16371] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ERP72 antibody (Green) at 1:500 dilution.

Immunohistochemistry-Paraffin: ERp72 Antibody [NBP2-16371] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Western Blot: ERp72 Antibody [NBP2-16371] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: ERp72 Antibody [NBP2-16371] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: ERp72 Antibody [NBP2-16371] - Wild-type (WT) and ERP72 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp72 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ERp72 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ERp72. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum lumen; Melanosome
• Marker: Endoplasmic Reticulum Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDIA4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (82%), Bovine (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ERp72 Antibody

• Endoplasmic reticulum resident protein 70
• Endoplasmic reticulum resident protein 72
• ER protein 70
• ERp70
• ERP70ER protein 72
• ERp72
• ERp-72
• ERP72EC 5.3.4.1
• intestinal-related)
• protein disulfide isomerase family A, member 4
• protein disulfide isomerase related protein (calcium-binding protein
• protein disulfide isomerase-associated 4
• protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PDIA4
• Uniprot:
  • P13667(Mouse)