ERP44 Antibody

Western Blot: ERP44 Antibody [NB100-93457] - Staining of K562 lysate with antibody at 0.2 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ERP44 Antibody Summary

• Description: Novus Biologicals Goat ERP44 Antibody (NB100-93457) is a polyclonal antibody validated for use in WB and ELISA. Anti-ERP44 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EQKDSDNYRVFER corresponding to internal region according to NP_055866.1.
• Epitope: C-EQKDSDNYRVFER
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERP44
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 0.6 ug/mL
• Application Notes: WB: Approx. 47 kDa band observed in K562 lysates (calculated MW of 47.0 kDa band according to NP_055866.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ERP44 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERP44 Antibody

• endoplasmic reticulum protein 44
• endoplasmic reticulum resident protein 44 kDa
• ER protein 44
• ERp44
• KIAA0573endoplasmic reticulum resident protein 44
• PDIA10
• protein disulfide isomerase family A, member 10
• thioredoxin domain containing 4 (endoplasmic reticulum)
• Thioredoxin domain-containing protein 4
• TXNDC4

Background

ERP44 mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protei

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERP44 Antibody (NB100-93457)(1)

Publication using NB100-93457

• Anelli T, Ceppi S, Bergamelli L et al. Sequential steps and checkpoints in the early exocytic compartment during secretory IgM biogenesis. EMBO J 2007-10-03 [PMID: 17805346]

Bioinformatics

• Gene Symbol: ERP44
• Entrez:
  • 23071(Human)
  • 76299(Mouse)
  • 298066(Rat)
• Uniprot:
  • Q9BS26(Human)