ITPR2 Antibody

Images

 
Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours ...read more
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ITPR2 Antibody Summary

Immunogen
Peptide with sequence C-PIEESNILSPVQD corresponding to internal region according to NP_002214.2.
Specificity
No cross-reactivity expected with other types of ITPR.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITPR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 28-30 kDa band in human heart lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 308 kDa band according to NP_002214.2. The 28-30 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Kidney, Skin and Heart.
Control Peptide
ITPR2 Protein (NB100-2466PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB100-2466 in the following applications:

Publications
Read Publications using
NB100-2466 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ITPR2 Antibody

  • inositol 14,5-triphosphate receptor, type 2
  • inositol 14,5-trisphosphate receptor type 2
  • insP3R2
  • IP3 receptor isoform 2
  • IP3 receptor
  • IP3R 2
  • IP3R2
  • Type 2 inositol 14,5-trisphosphate receptor
  • Type 2 InsP3 receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ITPR2 Antibody (NB100-2466)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(4)
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Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 5 of 5.
Publications using NB100-2466 Applications Species
Liu F, Li Zf, Wang Zy, Wang L. Role of subcellular calcium redistribution in regulating apoptosis and autophagy in cadmium-exposed primary rat proximal tubular cells. J. Inorg. Biochem. Sep 14 2016 [PMID: 27665314] (WB) WB
Wang H, Wang ZK, Jiao P et al. Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead Toxicology 2015 Apr 25 [PMID: 25921245] (WB, Rat) WB Rat
Ljubojevic S, Radulovic S, Leitinger G et al. Early Remodelling of Perinuclear Ca2+ Stores and Nucleoplasmic Ca2+ Signalling During the Development of Hypertrophy and Heart Failure. Circulation. 2014 Jun 13 [PMID: 24928680] (ICC/IF, WB, Human, Mouse)

Details:
In this study involving hypertrophy and heart failure models, goat polyclonal anti-IP3R2 antibody from Novus Biologicals (ITPR2 antibody, NB100-2466) was used for ICC-IF immunostaining of Human and Mouse cardiomyocytes isolated from nonfailing and failing hearts (Figure 2 B). Antibody also used for WB in human heart homogenate (Figure S5C) and for immunogold labeling/transmission immuno-electron microscopy in mouse hearts (Figure 2 D).
ICC/IF, WB Human, Mouse
Harr MW, McColl KS, Zhong F et al. Glucocorticoids downregulate Fyn and inhibit IP(3)-mediated calcium signaling to promote autophagy in T lymphocytes Autophagy 2010 Oct [PMID: 20814235] (WB, Mouse) WB Mouse
Futatsugi A, Nakamura T, Yamada MK et al. IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism. Science 2005 Sep 30 [PMID: 16195467]

Review for ITPR2 Antibody (NB100-2466) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2466:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/22/2010
View

Summary

ApplicationWestern Blot
Sample Testedmouse cell line, Sample Amount: 80ug
SpeciesMouse
CommentsThere are very few IP3 Receptor Type 2 antibodies out there that are specific only for Type 2 and work well in western blots. We had resorted to using a non-commercial antibody but when that ran out I had to find another antibody with the hope that something would work as well. I was SO surprised when this Novus antibody worked even BETTER than our non-commercial source! This antibody rocks!! It works best if you use ECL Plus right away.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in T-TBS, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/500, Incubation Dilution Buffer: 5% milk in T-TBS, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-goat HRP (from Santa Cruz cat. # sc2056), Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 2 to 5 minutes, Specific Bands: 300 kDa, Non-Specific Bands: 200 kDa

Comments

CommentsThere are very few IP3 Receptor Type 2 antibodies out there that are specific only for Type 2 and work well in western blots. We had resorted to using a non-commercial antibody but when that ran out I had to find another antibody with the hope that something would work as well. I was SO surprised when this Novus antibody worked even BETTER than our non-commercial source! This antibody rocks!! It works best if you use ECL Plus right away.

Product General Protocols

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Video Protocols

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FAQs for ITPR2 Antibody (NB100-2466) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
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1
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Anonymous
01/22/2010
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol ITPR2
Entrez
Uniprot