Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. Primary antibody applied at 1:500 overnight at 4degC, secondary donkey anti-goat applied for 2 hours ...read more
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: ITPR2 Antibody [NB100-2466] - (3.75ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB: Preliminary experiments gave an approx. 28-30 kDa band in human heart lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 308 kDa band according to NP_002214.2. The 28-30 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: Paraffin embedded Human Kidney, Skin and Heart. Use in Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature.
Rat reactivity reported in scientific literature (PMID: 25921245). Mouse reactivity reported scientific literature (PMID: 20814235). Predicted cross-reactivity based on sequence identity: Canine, Bovine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for ITPR2 Antibody
inositol 14,5-triphosphate receptor, type 2
inositol 14,5-trisphosphate receptor type 2
IP3 receptor isoform 2
Type 2 inositol 14,5-trisphosphate receptor
Type 2 InsP3 receptor
Inositol 1,4,5-triphosphate (IP3) receptors are a form of ligand-gated ion channels that are activated by cytosolic Ca2+ and IP3. They are localized to intracellular membranes, such as the endoplasmic reticulum, and mediate the mobilization of intracellular Ca2+ stores. IP3 receptors play an important role in intracellular Ca2+ signaling in a variety of cell types. There are three IP3 receptor subtypes; IP3R1, IP3R2 and IP3R3, which exist as homo- and heterotetramers. All subtypes are closely associated with calmodulin and FK506-binding protein and are modulated through phosphorylation by PKA, PKC, PKG and CaMKII.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Kaufman AM Non-canonical members of circuits: A role for the locus coeruleus in reward related place field plasticity, and investigating differences in astrocyte calcium signaling between hippocampal layers Thesis 2020-01-01 (ICC/IF, Mouse)
Review for ITPR2 Antibody (NB100-2466) (1)
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Mouse.
Reviews using NB100-2466:
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reviewed by: Verified Customer
mouse cell line, Sample Amount: 80ug
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