ERMAP Antibody (767505)

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB49281, filled histogram) or isotype control antibody (Catalog # MAB002, open histo­gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 767505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ERMAP Antibody (767505) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ERMAP
  His30-Ala155
  Accession # Q96PL5
• Specificity: Detects human ERMAP in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERMAP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERMAP Antibody (767505)

• ERMAP
• erythroblast membrane-associated protein (RD and SC blood groups)
• erythroblast membrane-associated protein (Scianna blood group)
• erythroblast membrane-associated protein
• erythroid membrane-associated protein
• hERMAP
• MGC118810
• MGC118811
• PRO2801
• Radin blood group (Rd)
• Radin blood group antigen
• Radin blood group
• RD
• RDMGC118812
• SC
• Scianna blood group (Sc)
• Scianna blood group antigen
• Scianna blood group
• SCMGC118813

Background

ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60-66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors, and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30-155) that contains one V-type Ig-like domain (aa #30-140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103-475. Over aa 30-155, human ERMAP shares 56% aa sequence identity with mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERMAP
• Uniprot:
  • Q96PL5()