Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: ErbB4/Her4 Antibody [NBP1-33120] - MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: ErbB4/Her4 Antibody [NBP1-33120] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit ...read more
Western Blot: ErbB4/Her4 Antibody [NBP1-33120] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] ...read more
Immunoprecipitation: ErbB4/Her4 Antibody [NBP1-33120] - Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 ug of Her4 / ErbB4 antibody [C1C3]. Western blot analysis was performed using ...read more
147 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NBP1-33120 in the following applications:
erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.