ErbB4/Her4 Antibodies

NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="ErbB4/Her4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB1131) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="ErbB4/Her4 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human ErbB4/Her4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1131) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF017). A specific band was detected for ErbB4/Her4 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for ErbB4/Her4 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of T47D human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ErbB4/Her4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ErbB4/Her4 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for ErbB4/Her4 at approximately 180 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for ErbB4/Her4 at approximately 180 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1131-ER) binds to immobilized Recombinant Human NRG1-beta 1/ HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) coated at 100 ng/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Human NRG1-beta 1/HRG1-beta 1 Monoclonal Antibody (Catalog # MAB11312). At 5 µg/mL, this antibody will block >75% of the binding." class="big_lightbox"> 1131-ER) binds to immobilized Recombinant Human NRG1-beta 1/ HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) coated at 100 ng/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Human NRG1-beta 1/HRG1-beta 1 Monoclonal Antibody (Catalog # MAB11312). At 5 µg/mL, this antibody will block >75% of the binding." alt="In a functional ELISA, Recombinant Human ErbB4/Her4 Fc Chimera (Catalog # 1131-ER) binds to immobilized Recombinant Human NRG1-beta 1/ HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) coated at 100 ng/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Mouse Anti-Human NRG1-beta 1/HRG1-beta 1 Monoclonal Antibody (Catalog # MAB11312). At 5 µg/mL, this antibody will block >75% of the binding." class="big_thumb" />

DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY998) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY998) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human ErbB4/Her4 protein was serially diluted 2-fold and captured by Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB11314) coated on a Clear Polystyrene Microplate ( DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY998) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

MAB11314) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB11314) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human ErbB4/Her4 protein was serially diluted 2-fold and captured by Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (MAB11314) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ErbB4/Her4 Monoclonal Antibody (Catalog # MAB11313) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />