ErbB4/Her4 Antibody (S07-2H1)

Western Blot: ErbB4/Her4 Antibody (S07-2H1) [NBP3-15027] - Western blot detection of ErbB4/Her4 in Hela, A549, HL-60, U2OS, C6 cell lysates using ErbB4/Her4 Rabbit mAb (1:1000 diluted). Predicted band size: 147kDa. Observed band size: 180kDa.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: S07-2H1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ErbB4/Her4 Antibody (S07-2H1) Summary

• Description: Novus Biologicals Rabbit ErbB4/Her4 Antibody (S07-2H1) (NBP3-15027) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human ErbB4/Her4 (Uniprot#: Q15303)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERBB4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for ErbB4/Her4 Antibody (S07-2H1)

• avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• EC 2.7.10
• EC 2.7.10.1
• ErbB4
• HER4
• HER4MGC138404
• p180erbB4
• Proto-oncogene-like protein c-ErbB-4
• receptor tyrosine-protein kinase erbB-4
• Tyrosine kinase-type cell surface receptor HER4
• v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4
• v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)

Background

erbB4/HER4 is a receptor tyrosine kinase that belongs to the human epidermal growth factor receptor (EGFR) family. erbB4/HER4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum (1). erbB4/HER4 is activated by neuregulins (NRG), betacellulin (BTC), and heparin-binding EGF-like growth factor (2). Neuregulins regulate the expression of ligand- and voltage-gated channels in neurons and skeletal muscle by the activation of ErbB 1-4. The erbB4/HER4 receptor is unique among these receptors because its C-terminal tail binds to a protein motif known as the PDZ domain (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERBB4