ERAB Antibody (OTI10B4) - Azide and BSA Free

Western Blot: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAB.

Immunocytochemistry/Immunofluorescence: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAB.

Immunohistochemistry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of paraffin-embedded liver tissue using anti-ERAB mouse monoclonal antibody.

Flow Cytometry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Analysis of Hela cells, using anti-ERAB antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ERAB monoclonal antibody.

Immunohistochemistry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERAB mouse monoclonal antibody.

Immunohistochemistry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-ERAB mouse monoclonal antibody.

Immunohistochemistry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of paraffin-embedded Kidney tissue using anti-ERAB mouse monoclonal antibody.

Immunohistochemistry: ERAB Antibody (OTI10B4) - Azide and BSA Free [NBP2-70871] - Staining of paraffin-embedded pancreas tissue using anti-ERAB mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI10B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ERAB Antibody (OTI10B4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ERAB Antibody (OTI10B4) - Azide and BSA Free (NBP2-02118) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HSD17B10(NP_004484) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSD17B10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 26.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ERAB Antibody (OTI10B4) - Azide and BSA Free

• 17-beta-HSD 10
• 17-beta-hydroxysteroid dehydrogenase 10
• 17b-HSD10
• 3-hydroxy-2-methylbutyryl-CoA dehydrogenase
• ABAD
• amyloid-beta peptide binding alcohol dehydrogenase
• CAMR
• DUPXp11.22
• EC 1.1.1.178
• EC 1.1.1.35
• Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
• ERAB3-hydroxyacyl-CoA dehydrogenase type-2
• HADH2AB-binding alcohol dehydrogenase
• hydroxyacyl-Coenzyme A dehydrogenase, type II, hydroxyacyl-Coenzyme Adehydrogenase, type II
• hydroxysteroid (17-beta) dehydrogenase 10
• mental retardation, X-linked, syndromic 10
• MHBD
• Mitochondrial ribonuclease P protein 2,3-hydroxyacyl-CoA dehydrogenase type II
• Mitochondrial RNase P protein 2
• MRPP2HCD2
• MRX17
• MRX31
• MRXS10
• SCHAD
• SDR5C1
• short chain dehydrogenase/reductase family 5C, member 1
• short chain L-3-hydroxyacyl-CoA dehydrogenase type 2
• short chain type dehydrogenase/reductase XH98G2
• Short-chain type dehydrogenase/reductase XH98G2
• Type II HADH
• XH98G2

Background

ERAB encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSD17B10