HADH Antibody (4B5)

Images

 
Western Blot: HADH Antibody (4B5) [H00003033-M01] - HADHSC monoclonal antibody (M01), clone 4B5 Analysis of HADHSC expression in HepG2.
Immunocytochemistry/ Immunofluorescence: HADH Antibody (4B5) [H00003033-M01] - Analysis of monoclonal antibody to HADHSC on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: HADH Antibody (4B5) [H00003033-M01] - Analysis of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: HADH Antibody (4B5) [H00003033-M01] - Analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody (M01), clone 4B5.Lane 1: HADH transfected lysate(34.3 KDa).Lane 2: ...read more
Immunoprecipitation: HADH Antibody (4B5) [H00003033-M01] - Analysis of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH MaxPab rabbit polyclonal ...read more
ELISA: HADH Antibody (4B5) [H00003033-M01] - Detection limit for recombinant GST tagged HADHSC is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, IP
Clone
4B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HADH Antibody (4B5) Summary

Immunogen
HADHSC (NP_005318 205 a.a. - 314 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK
Localization
Mitochondrial; mitochondrial matrix.
Specificity
HADHSC - L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HADH
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publications using H00003033-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HADH Antibody (4B5)

  • EC 1.1.1
  • EC 1.1.1.35
  • HADH1
  • HADHSChydroxyacyl-Coenzyme A dehydrogenase
  • HCDH
  • hydroxyacyl-CoA dehydrogenase
  • L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
  • Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
  • MGC8392
  • mitochondrial
  • MSCHAD
  • SCHADHHF4

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HADH Antibody (H00003033-M01)(3)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Diseases for HADH Antibody (H00003033-M01)

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Bioinformatics

Gene Symbol HADH
Entrez
OMIM
Uniprot