SDPR Antibody

Western blot shows lysates of human heart and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SDPR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5759) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SDPR at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SDPR Antibody Summary

• Immunogen: E. coli-derived recombinant human SDPR
  Gly2-Val180
  Accession # O95810
• Specificity: Detects human and mouse SDPR in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SDPR
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SDPR Antibody

• cavin-2
• Phosphatidylserine-binding protein
• PS-p68
• PS-p68serum deprivation response (phosphatidylserine-binding protein)
• SDPR
• SDR
• SDRphosphatidylserine binding protein
• serum deprivation response (phosphatidylserine binding protein)
• serum deprivation response
• serum deprivation-response protein

Background

SDPR (Serum deprivation response gene; also SDR and PS-p68) is a 68‑70 kDa member of the PTRF/SDPR family of proteins. It is expressed in striated muscle, platelets, and fibroblasts, and is cytosolic in location. SDPR is known to bind phosphatidylserine and form complexes with both PKC alpha and PTRF, targeting them to caveolae. Human SDPR is 425 amino acids (aa) in length. It contains an N-terminal acetylglycine, three coiled-coil regions (aa 61‑82, 125‑154, and 210‑268), and two serine phosphorylation sites at positions 287 and 293. Over aa 1‑180, human SDPR shares 91% aa identity with mouse SDPR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SDPR Antibody (AF5759)(2)

Publications using AF5759

• E Seemann, M Sun, S Krueger, J Tröger, W Hou, N Haag, S Schüler, M Westermann, CA Huebner, B Romeike, MM Kessels, B Qualmann Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination Elife, 2017;6(0):. 2017 [PMID: 29202928] (Western Blot, Mouse)
• B Hansson, C Rippe, D Kotowska, S Wasserstro, J Säll, O Göransson, K Swärd, KG Stenkula Rosiglitazone drives cavin-2/SDPR expression in adipocytes in a CEBP?-dependent manner PLoS ONE, 2017;12(3):e0173412. 2017 [PMID: 28278164] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SDPR
• Uniprot:
  • O95810(Human)