ER alpha/NR3A1 Antibody - BSA Free

Western Blot: ER alpha/NR3A1 Antibody [NB200-347] - Detection of Human Estrogen Receptor Alpha by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody NB200-347 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: ER alpha/NR3A1 Antibody [NB200-347] - Detection of human ER alpha/NR3A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ER alpha/NR3A1 antibody NB200-347 (lot 3) used for IP at 6 ug per reaction. ER alpha/NR3A1 was also immunoprecipitated by previous lot of this antibody (lot 2). For blotting immunoprecipitated ER alpha/NR3A1, NB200-347 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ER alpha/NR3A1 Antibody - BSA Free Summary

• Description: This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-158, NB100-1567, NB100-58824, NB100-97831, NB200-171, NB100-2254, NB100-58826, NB600-233, NB100-314, NB100-77315, NB100-77315
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 595) of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:5000-1:15000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ER alpha/NR3A1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB500-158, NB100-1567, NB100-58824, NB100-97831, NB200-171, NB100-2254, NB100-58826, NB600-233, NB100-314, NB100-77315, NB100-77315

Alternate Names for ER alpha/NR3A1 Antibody - BSA Free

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER alpha/NR3A1 Antibody (NB200-347)(5)

Publications using NB200-347

• Ha NH, Nair VS, Reddy DN et al. Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes. Cancer Res 2011-12-01 [PMID: 22006997]
• Di Leva G, Gasparini P, Piovan C et al. MicroRNA cluster 221-222 and estrogen receptor alpha interactions in breast cancer. J Natl Cancer Inst 2010-05-01 [PMID: 20388878]
• Ohshiro K, Mudvari P, Meng QC et al. Identification of a novel estrogen receptor-alpha variant and its upstream splicing regulator. Mol Endocrinol 2010-05-01 [PMID: 20304996]
• Popov VM, Zhou J, Shirley LA et al. The cell fate determination factor DACH1 is expressed in estrogen receptor-alpha-positive breast cancer and represses estrogen receptor-alpha signaling. Cancer Res 2009-07-01 [PMID: 19605405]
• Zheng X, Chu F, Chou PM et al. Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism. Am J Physiol Cell Physiol 2009-01-01 [PMID: 18971393]

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
• Uniprot:
  • P03372()