ER alpha/NR3A1 [p Ser118] Antibody (SC05-87)

Western Blot: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Western blot analysis of ER alpha/NR3A1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goa

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Staining phospho-Estrogen Receptor alpha(S118) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ER alpha/NR3A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Analysis of Estrogen Receptor alpha(phospho S118) on MCF-7 cell lysates using anti- phospho-Estrogen Receptor alpha(S118) antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Staining phospho-Estrogen Receptor alpha(S118) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Staining phospho-Estrogen Receptor alpha(S118) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Analysis of paraffin-embedded mouse placenta tissue using anti- phospho-Estrogen Receptor alpha(S118) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ER alpha/NR3A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) [NBP2-67575] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ER alpha/NR3A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

ER alpha/NR3A1 [p Ser118] Antibody (SC05-87) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser118 of Human ER alpha/NR3A1 aa 110-154 / 595. (SwissProt: P03372 Human; SwissProt: P19785 Mouse)
• Modification: p Ser118
• Localization: Nucleus, Cytoplasm, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ESR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ER alpha/NR3A1 [p Ser118] Antibody (SC05-87)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1