ER alpha/NR3A1 Antibody (h-151) - BSA Free

Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IP
• Clone: h-151
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ER alpha/NR3A1 Antibody (h-151) - BSA Free Summary

• Description: Novus Biologicals Mouse ER alpha/NR3A1 Antibody (h-151) - BSA Free (NB120-13538) is a monoclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide derived from the sequence of human Estrogen Receptor, conjugated to KLH
• Localization: Nuclear
• Specificity: Binding specificity was confirmed in peptide inhibition studies with the intact protein. This does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 15 ug/ml
  • Western Blot 1:250
• Application Notes: Detects a band of ~67 kDa by Western blot.

Reactivity Notes

Human and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for ER alpha/NR3A1 Antibody (h-151) - BSA Free

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
  • 13982(Mouse)
  • 24890(Rat)
• Uniprot:
  • P03372()