ER alpha/NR3A1 Antibody (h-151)

Images

 
Western Blot: ER alpha/NR3A1 Antibody (h-151) [NB120-13538] - Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
h-151
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ER alpha/NR3A1 Antibody (h-151) Summary

Immunogen
Synthetic peptide derived from the sequence of human Estrogen Receptor, conjugated to KLH
Localization
Nuclear
Specificity
Binding specificity was confirmed in peptide inhibition studies with the intact protein. This does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 15 ug/ml
Application Notes
Detects a band of ~67 kDa by Western blot.

Reactivity Notes

Human and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for ER alpha/NR3A1 Antibody (h-151)

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

The Estrogen receptors (ER), mainly ERalpha and ERbeta, are members of the nuclear steroid hormone receptor family and are composed of three domains, an N-terminal domain required for maximal transactivation function (TAF), a DNA binding domain, and a steroid binding domain. Estrogen has pleiotropic effects on the growth, development, and maintenance of diverse tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ER alpha/NR3A1 Antibody (NB120-13538) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-13538AF405
Alexa Fluor 488 Labeled NB120-13538AF488
Alexa Fluor 647 Labeled NB120-13538AF647
Alexa Fluor 700 Labeled NB120-13538AF700
DyLight 350 Labeled NB120-13538UV
DyLight 405 Labeled NB120-13538V
DyLight 488 Labeled NB120-13538G
DyLight 680 Labeled NB120-13538FR
DyLight 755 Labeled NB120-13538IR

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Bioinformatics

Gene Symbol ESR1
Entrez
Uniprot