ER alpha/NR3A1 Antibody (ER505) - Azide and BSA Free

Western Blot: ER alpha/NR3A1 Antibody (ER505) - Azide and BSA Free [NBP2-33106] - Western blot analysis of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor Ab (ER505).

• Reactivity: Hu
• Applications: WB
• Clone: ER505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

ER alpha/NR3A1 Antibody (ER505) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32733).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human ER alpha/NR3A1 alpha protein (aa2-185) (Uniprot: P03372)
• Localization: Nuclear
• Marker: Marker of Estrogen Dependence
• Specificity: This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded breast carcinomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2-4 ug/mL
• Application Notes: Optimal dilution for a specific application should be determined.
  Western Blot: 1-2ug/ml for 2 hours at RT.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for ER alpha/NR3A1 Antibody (ER505) - Azide and BSA Free

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1