ER alpha/NR3A1 Antibody (C-542) - BSA Free

Western Blot: ER alpha/NR3A1 Antibody (C-542) [NBP1-19318] - Analysis of Estrogen Receptor alpha mAb: Lane 1: MWM, Lane 2: Estrogen Receptor 1 (GST-tagged ER1 protein control runs at 92 kDa).

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC, IP
• Clone: C-542
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ER alpha/NR3A1 Antibody (C-542) - BSA Free Summary

• Description: Novus Biologicals Mouse ER alpha/NR3A1 Antibody (C-542) - BSA Free (NBP1-19318) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-ER alpha/NR3A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of human Estrogen Receptor alpha.
• Specificity: Estrogen Receptor alpha (C-542)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Detects a band of 66.3 kDa by Western blot. Recommended dilutions are for colorimetric detection.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for ER alpha/NR3A1 Antibody (C-542) - BSA Free

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ER alpha/NR3A1 Antibody (NBP1-19318)(1)

Publication using NBP1-19318

• R Tamosiunie, O Manouvakho, P Mesange, T Saito, J Qian, M Sanyal, YC Lin, LP Nguyen, A Luria, AB Tu, JM Sante, M Rabinovitc, DJ Fitzgerald, BB Graham, A Habtezion, NF Voelkel, L Aurelian, MR Nicolls A Dominant Role for Regulatory T Cells in Protecting Females Against Pulmonary Hypertension Circ. Res., 2018-03-15;0(0):. 2018-03-15 [PMID: 29545367]

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
  • 13982(Mouse)
  • 24890(Rat)
• Uniprot:
  • P03372()