ER alpha/NR3A1 Antibody (1F3)

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Sample (30 ug of whole cell lysate) A: MCF7 7. 5% SDS PAGE, antibody diluted at 1:500.

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Whole cell extract (60 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Product Discontinued

ER alpha/NR3A1 Antibody (1F3) Summary

• Immunogen: The region encoding amino acids 1-190 expressed in E. coli.
• Localization: Nuclear
• Specificity: This antibody will recognize human ER alpha.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1-5 ug/ml
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 14972507).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for ER alpha/NR3A1 Antibody (1F3)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

The Estrogen Receptor (ER) is a 65a protein and a member of the steroid family of nuclear receptors. ER is a ligand-activated transcription factor and, when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) sequences. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1
• Entrez:
  • 2099(Human)
• Uniprot:
  • P03372()