![Immunocytochemistry/Immunofluorescence: Ephrin-B Antibody [NBP2-57916] - Staining of human cell line PC-3 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57916-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ephrin-B Antibody [NBP2-57916] - Staining of human cell line PC-3 shows localization to plasma membrane.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Ephrin-B1 Antibody - BSA Free (NBP2-57916) is a polyclonal antibody validated for use in ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: VGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGA |
| Predicted Species | Mouse (93%), Rat (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EFNB1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Ephrin-B1 Antibody (NBP2-57916)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EFNB1 |
![Western Blot EphA1 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA1_AF638_Western_Blot_17606.jpg)
![Immunocytochemistry EphA1 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA1_AF638_Immunocytochemistry__Immunofluorescence_20119.jpg)
![Western Blot Ephrin-B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ephrin-B2_AF496_Western_Blot_11024.jpg)
![Immunocytochemistry Ephrin-B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ephrin-B2_AF496_Immunocytochemistry_6513.jpg)
![Flow Cytometry Ephrin-B2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphrinB2_AF496_Flow_Cytometry_18997.jpg)
![Western Blot Ephrin-B3 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphrinB3_AF395_Western_Blot_16885.jpg)
![Immunocytochemistry EphB3 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB3_AF432_Immunocytochemistry_10108.jpg)
![Western Blot EphB3 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB3_AF432_Western_Blot_23994.jpg)
![Western Blot EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Knockout_Validated_21256.jpg)
![Immunohistochemistry EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Immunohistochemistry_7327.jpg)
![Flow Cytometry EphA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphA2_AF3035_Flow_Cytometry_20077.jpg)
![Immunohistochemistry Ephrin-A4 Antibody (135305) [Unconjugated]](https://images.novusbio.com/images2/EphrinA4_MAB369_Immunohistochemistry_21487.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")