HAF019). A specific band was detected for Ephrin-B1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Ephrin-B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF473) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin-B1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF473-SP | |||
HAF005). A specific band was detected for Ephrin-B1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse lung tissue and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Ephrin-B1 Monoclonal Antibody (Catalog # MAB4731) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Ephrin-B1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4731-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57916-25ul | |||
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![Western Blot: Ephrin-B Antibody [NBP1-59211] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Western-Blot-NBP1-59211-img0003.jpg "Western Blot: Ephrin-B Antibody [NBP1-59211] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Western Blot: Ephrin-B Antibody [NBP1-59211] - Human kidney lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Western-Blot-NBP1-59211-img0002.jpg)
![Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/EPH-Receptor-B1-[p-Tyr317]-Antibody-Western-Blot-NB300-275-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.")
![Immunocytochemistry/Immunofluorescence: Ephrin-B Antibody [NBP2-57916] - Staining of human cell line PC-3 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57916-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ephrin-B Antibody [NBP2-57916] - Staining of human cell line PC-3 shows localization to plasma membrane.")
![Western Blot: Ephrin-B1 [p Tyr331] Antibody [NB300-276] - Analysis of EphB1 [p Tyr331] Antibody in rat testes lysate using this antibody at 1:1000.](http://images.novusbio.com/fullsize/EphB1-[p-Tyr331]-Antibody-Western-Blot-NB300-276-img0003.jpg "Western Blot: Ephrin-B1 [p Tyr331] Antibody [NB300-276] - Analysis of EphB1 [p Tyr331] Antibody in rat testes lysate using this antibody at 1:1000.")
![Western Blot: EphB1 [p Tyr298] Antibody [NB300-274] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr298 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/EPH-Receptor-B1-[p-Tyr298]-Antibody-Western-Blot-NB300-274-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EphB1 [p Tyr298] Antibody [NB300-274] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr298 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: Ephrin B Overexpression Lysate (Adult Normal) [NBL1-10146] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ephrin B.](http://images.novusbio.com/fullsize/Ephrin-B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10146-img0002.jpg "Western Blot: Ephrin B Overexpression Lysate (Adult Normal) [NBL1-10146] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ephrin B.")
