EphB4 Overexpression Lysate (Native)


Western Blot: EPH Receptor B4 Overexpression Lysate (Adult Normal) [NBL1-10293] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EPH Receptor B4.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

EphB4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC208559 and based on accession number NM_004444. The protein contains a C-terminal DDK tag.
Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
106.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for EphB4

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EphB4 Overexpression Lysate (Native)

  • EC 2.7.10
  • EC
  • EPH receptor B4
  • EphB4
  • ephrin type-B receptor 4
  • hepatoma transmembrane kinase
  • Htk
  • HTKephrin receptor EphB4
  • Mdk2
  • Myk1
  • soluble EPHB4 variant 1
  • soluble EPHB4 variant 2
  • soluble EPHB4 variant 3
  • TYRO11
  • Tyrosine-protein kinase receptor HTK
  • Tyrosine-protein kinase TYRO11


Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for EphB4 Lysate (NBL1-10293) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol EPHB4

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