EphB4 Antibody (VEB4-7E4)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IA, ICC/IF, IHC, IHC-Fr
Clone
VEB4-7E4
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

EphB4 Antibody (VEB4-7E4) Summary

Immunogen
Mouse Tyrosine-protein kinase receptor HTK
Specificity
Mouse Ephrin Type-B Receptor 4
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EPHB4
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

The monoclonal antibody VEB4-7E4 marks venous endothelial cells, but not arterial endothelial cells in B16 melanoma cells.
Publications
Read Publication using
NBP1-49607 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, containing 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for EphB4 Antibody (VEB4-7E4)

  • EC 2.7.10
  • EC 2.7.10.1
  • EPH receptor B4
  • EphB4
  • ephrin type-B receptor 4
  • hepatoma transmembrane kinase
  • Htk
  • HTKephrin receptor EphB4
  • Mdk2
  • Myk1
  • soluble EPHB4 variant 1
  • soluble EPHB4 variant 2
  • soluble EPHB4 variant 3
  • Tyro11
  • Tyrosine-protein kinase receptor HTK
  • Tyrosine-protein kinase TYRO11

Background

The monoclonal antibody VEB4-7E4 recognizes mouse Ephrin type-B receptor 4 (EphB4), an ~110 kD protein. Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors and ephrins are membrane proteins. They are classified into 2 broad subclasses, namely A and B, according to structural homologies and binding specificities. Eph receptors are tyrosine kinases, which autophosphorylate upon binding to their cognate ephrin ligands. Eph receptors and ephrins are frequently expressed in reciprocal patterns that correlate with cellular boundaries during embryonic development. The interaction between EphB4 and its ligand, Ephrin-B2, plays an important role in cell-cell, cell-extracellular matrix interactions as well as in cell migration, adhesion and proliferation. During the early stages of vascular development, EphB4 is specifically expressed in venous endothelium, whereas Ephrin-B2 is expressed in arterial endothelium. In mouse embryo's, EphB4 and Ephrin-B2 are essential for embryonic heart development and angiogenesis. In adult microcirculation, EphB4 is not a ubiquitous marker of arterial/venous polarity, but is expressed along both venules and arterioles. Furthermore, EphB4 is upregulated by endothelial cells along blind-ended capillary sprouts versus connecting capillaries. As such, EphB4 is thought to play a role in the patterning of new vessels during angiogenesis. EphB4 is also expressed in a variety of tumor cells, like gastrointestinal, prostate, bladder, breast, liver, lung and ovarian cancers, as well as leukemia, mesothelioma, malignant breast tumors and melanoma. Reduction of EphB4 activity accelerated tumorigenesis in colon and rectum. In head and neck squamous cell carcinoma and endometrial carcinoma, overexpression of EphB4 is inversely related to a poor prognosis. However, in mesothelioma, up-regulation of EphB4 resulted in growth of the tumor. Besides the essential expression of EphB4, coexpression of other EphB4 family members or EphB-ligands may affect tumor cell viability and proliferation as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB4 Antibody (NBP1-49607)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on EphB4.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

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Bioinformatics

Gene Symbol EPHB4
Entrez