EphB4 Antibody (117808)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
117808
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

EphB4 Antibody (117808) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB4
Leu16-Ala539
Accession # P54761
Specificity
Detects mouse EphB4 in direct ELISAs and Western blots. Shows less than 10% cross‑reactivity with recombinant mouse (rm) EphA8 and no cross-reactivity with recombinant human EphA1, rmEphA2, A3, A4, A6, A7, B6, or recombinant rat EphA5.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
EPHB4
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB446 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB4 Antibody (117808)

  • EC 2.7.10
  • EC 2.7.10.1
  • EPH receptor B4
  • EphB4
  • ephrin type-B receptor 4
  • hepatoma transmembrane kinase
  • Htk
  • HTKephrin receptor EphB4
  • Mdk2
  • Myk1
  • soluble EPHB4 variant 1
  • soluble EPHB4 variant 2
  • soluble EPHB4 variant 3
  • Tyro11
  • Tyrosine-protein kinase receptor HTK
  • Tyrosine-protein kinase TYRO11

Background

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin-B2 and ephrin-B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB4 Antibody (MAB446)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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(1)
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Bioinformatics

Gene Symbol EPHB4
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