EphB3 Antibody (647354) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: Flow
• Clone: 647354
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

EphB3 Antibody (647354) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EphB3
  Leu38-Ala550
  Accession # P54753
• Specificity: Detects human EphB3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) EphA3, rhEphA4, EphB2, or recombinant mouse EphB3 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHB3

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB3 Antibody (647354) [Alexa Fluor® 750]

• Cek10
• EC 2.7.10
• EC 2.7.10.1
• EK2
• EPH receptor B3
• EphB3
• EPH-like kinase 2
• EPH-like tyrosine kinase-2
• ephrin type-B receptor 3
• ETK2Hek2
• Hek2
• human embryo kinase 2
• Mdk5
• Sek4
• Tyro6
• Tyrosine-protein kinase TYRO6

Background

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 110-130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 15).

1. Pasquale, E.B. (2008) Cell 133:38.
2. Bohme, B. et al. (1993) Oncogene 8:2857.
3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
10. Birgbauer, E. et al. (2000) Development 127:1231.
11. Alfaro, D. et al. (2008) Immunology 125:131.
12. Risley, M. et al. (2009) Mech. Dev. 126:230.
13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
14. Batlle, E. et al. (2005) Nature 435:1126.
15. Holmberg, J. et al. (2006) Cell 125:1151.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHB3
• Uniprot:
  • P54753()