EphA1 Antibody


Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3034) followed by HRP-conjugated Anti-Goat ...read more
EphA1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3034) at 1.7 µg/mL overnight at 4 °C. ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready

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EphA1 Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse EphA1
Accession # AAH71215
Detects mouse EphA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human EphA1 and less than 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA6, and rmEphA7 is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA1 Antibody

  • EC 2.7.10
  • EC
  • EPH receptor A1
  • eph tyrosine kinase 1
  • EphA1
  • EPHephrin type-A receptor 1
  • EPHT
  • EPHT1erythropoietin-producing hepatoma amplified sequence
  • Esk
  • MGC163163
  • oncogene EPH
  • soluble EPHA1 variant 1
  • soluble EPHA1 variant 2
  • Tyrosine-protein kinase receptor EPH


EphA1, also known as Eph and Esk, is a member of the Eph receptor tyrosine kinase family and binds several Ephrin-A ligands. The A and B class Eph proteins share a common structural organization (1-4). The mouse EphA1 cDNA encodes a 977 amino acid (aa) precursor that includes a 24 aa signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5, 6). Within the ECD, mouse EphA1 shares 84% aa sequence identity with human EphA1, approximately 40% aa sequence identity with mouse EphA2, 3, 4, 6, 7, and 8, and 27% aa sequence identity with mouse EphA5. A splice variant of mouse EphA1 lacks the transmembrane segment and is predicted to exist as a soluble molecule (7). Membrane bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (7). Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (5, 7). It is expressed on CD4+ T cells but not on CD19+ B cells (8). On T cells, EphA1 induces Tyr phosphorylation of PYK2 and promotes chemokine-induced chemotaxis (8). EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness (2, 9, 10).

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Coulthard, M.G. et al. (2001) Growth Factors 18:303.
  6. Lickliter, J.D. et al. (1996) Proc. Natl. Acad. Sci. USA 93:145.
  7. Douville, E.M.J. et al. (1992) Mol. Cell. Biol. 12:2681.
  8. Aasheim, H-C. et al. (2005) Blood 105:2869.
  9. Iwase, T. et al. (1993) Biochim. Biophys. Res. Commun. 194:698.
  10. Hirai, H. et al. (1987) Science 238:1717.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol EPHA1