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EpCAM/TROP1 Antibody (60N5D8)

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Western Blot: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary ...read more
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - FFPE human colon stained with EpCAM antibody (1:200), peroxidase-conjugate and DAB chromogen.
Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) [NBP2-22381] - Analysis of HT-29 cells using EpCAM antibody at 0.2 ug/10^6 cells. this antibody was used for this test. Top picture shows no cell surface staining seen, ...read more

Product Details

Summary
Reactivity Hu, Po, Bv, MkSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
60N5D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22381AF350
Alexa Fluor 405
NBP2-22381AF405
Alexa Fluor 488
NBP2-22381AF488
Alexa Fluor 532
NBP2-22381AF532
Alexa Fluor 594
NBP2-22381AF594
Alexa Fluor 647
NBP2-22381AF647
Alexa Fluor 700
NBP2-22381AF700
Alexa Fluor 750
NBP2-22381AF750
Allophycocyanin
NBP2-22381APC
Biotin
NBP2-22381B
DyLight 350
NBP2-22381UV
DyLight 405
NBP2-22381V
DyLight 488
NBP2-22381G
DyLight 550
NBP2-22381R
DyLight 594
NBP2-22381DL594
DyLight 650
NBP2-22381C
DyLight 680
NBP2-22381FR
DyLight 755
NBP2-22381IR
FITC
NBP2-22381F
HRP
NBP2-22381H
PE
NBP2-22381PE
PerCP
NBP2-22381PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80722
100% Guarantee on every product

EpCAM/TROP1 Antibody (60N5D8) Summary

Immunogen
Synthetic peptide made to an internal portion of the human EpCAM/TROP1 protein (between amino acids 289-314) [UniProt P16422]
Predicted Species
Porcine (96%), Monkey (96%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Flow Cytometry 0.2-1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:400

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for EpCAM/TROP1 Antibody (60N5D8)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for EpCAM/TROP1 Antibody (NBP2-22381). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22381AF350
Alexa Fluor 405 NBP2-22381AF405
Alexa Fluor 488 NBP2-22381AF488
Alexa Fluor 532 NBP2-22381AF532
Alexa Fluor 594 NBP2-22381AF594
Alexa Fluor 647 NBP2-22381AF647
Alexa Fluor 700 NBP2-22381AF700
Alexa Fluor 750 NBP2-22381AF750
Allophycocyanin NBP2-22381APC
Biotin NBP2-22381B
DyLight 350 NBP2-22381UV
DyLight 405 NBP2-22381V
DyLight 488 NBP2-22381G
DyLight 550 NBP2-22381R
DyLight 594 NBP2-22381DL594
DyLight 650 NBP2-22381C
DyLight 680 NBP2-22381FR
DyLight 755 NBP2-22381IR
FITC NBP2-22381F
HRP NBP2-22381H
PE NBP2-22381PE
PerCP NBP2-22381PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol EPCAM