EpCAM/TROP1 Antibody (158206) [Unconjugated]

HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB9601, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ELISA(Cap)
• Clone: 158206
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EpCAM/TROP1 Antibody (158206) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
  Extracellular domain
• Specificity: Detects human EpCAM/TROP-1 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
• Application Notes: ELISA Detection: Human EpCAM/TROP-1 Biotinylated Antibody (Catalog number BAF960)
  Standard: Recombinant Human EpCAM/TROP-1 Fc Chimera (Catalog number 960-EP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EpCAM/TROP1 Antibody (158206) [Unconjugated]

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca²⁺ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -Actinin (5).

1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EpCAM/TROP1 Antibody (MAB9601)(17)

Publications using MAB9601

• Nana Lyu, Bernadette Pedersen, Elena Shklovskaya, Helen Rizos, Mark P. Molloy, Yuling Wang SERS characterization of colorectal cancer cell surface markers upon anti‐EGFR treatment Exploration (Beijing) 2022-06-01 [PMID: 37323700]
• Collins DP, Osborn MJ, Steer CJ. et al. Differentiation of immortalized human multi-lineage progenitor to alveolar type 2-like cells: angiotensin-converting enzyme 2 expression and binding of severe acute respiratory syndrome coronavirus 2 spike and spike 1 proteins Cytotherapy 8/1/2021 [PMID: 34551876]
• Fei Wu, Yanzi Gu, Bin Kang, Fabienne Heskia, Alexandre Pachot, Marc Bonneville et al. PD-L1 detection on circulating tumor-derived extracellular vesicles (T-EVs) from patients with lung cancer Translational Lung Cancer Research 2021-06-01 [PMID: 34295653]
• Ralph El Hallal, Nana Lyu, Yuling Wang Effect of Cetuximab-Conjugated Gold Nanoparticles on the Cytotoxicity and Phenotypic Evolution of Colorectal Cancer Cells Molecules 2021-01-22 [PMID: 33499047]
• Ping Wei, Fei Wu, Bin Kang, Xiaohua Sun, Fabienne Heskia, Alexandre Pachot et al. Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer Journal of Extracellular Vesicles 2020-09-01 [PMID: 32944195]
• Collins D, Hapke J, Aravalli R, Steer C In vitro Differentiation of TERT-Transfected Multi-Lineage Progenitor Cells (MLPC) into Immortalized Hepatocyte-Like Cells HMER 6/1/2020 [PMID: 32607015]
• Chen-Han Huang, Yong Il Park, Hsing-Ying Lin, Divya Pathania, Ki Soo Park, Maria Avila-Wallace et al. Compact and Filter-Free Luminescence Biosensor for Mobile in Vitro Diagnoses ACS Nano 2019-10-22 [PMID: 31461265] (Bioassay, N/A)
• R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy PLoS ONE, 2019-05-02;14(5):e0215990. 2019-05-02 [PMID: 31048929] (ELISA Detection, Human)
• Erik A. Rodriguez, Ye Wang, Jessica L. Crisp, David R. Vera, Roger Y. Tsien, Richard Ting New Dioxaborolane Chemistry Enables [(18)F]-Positron-Emitting, Fluorescent [(18)F]-Multimodality Biomolecule Generation from the Solid Phase Bioconjugate Chemistry 2016-05-18 [PMID: 27064381]
• Dokyoon Kim, Changlin Fu, Xuefeng B Ling, Zhongkai Hu, Guozhong Tao, Yingzhen Zhao et al. Pilot Application of Magnetic Nanoparticle-Based Biosensor for Necrotizing Enterocolitis Journal of Proteomics & Bioinformatics 2016-01-23 [PMID: 26798207] (ELISA Capture, Human)
• Glenn D, Lee K, Park H, Weissleder R, Yacoby A, Lukin M, Lee H, Walsworth R, Connolly C Single-cell magnetic imaging using a quantum diamond microscope. Nat Methods, 2015-06-22;12(8):736-8. 2015-06-22 [PMID: 26098019] (ELISA Development, Human)
• Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro. BMC Cancer, 2015-05-07;15(0):372. 2015-05-07 [PMID: 25947366] (ELISA Development (Capture), Human)
• Im H, Castro C, Shao H, Liong M, Song J, Pathania D, Fexon L, Min C, Avila-Wallace M, Zurkiya O, Rho J, Magaoay B, Tambouret R, Pivovarov M, Weissleder R, Lee H Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone. Proc Natl Acad Sci U S A, 2015-04-13;112(18):5613-8. 2015-04-13 [PMID: 25870273] (Immunoassay Development, Human)
• Xiangjun Zheng, Linan Jiang, Joyce Schroeder, Alison Stopeck, Yitshak Zohar Isolation of viable cancer cells in antibody-functionalized microfluidic devices Biomicrofluidics 2014-03-01 [PMID: 24803968]
• Xiangjun Zheng, Luthur Siu-Lun Cheung, Joyce A. Schroeder, Linan Jiang, Yitshak Zohar Cell receptor and surface ligand density effects on dynamic states of adhering circulating tumor cells Lab on a Chip 2011-10-21 [PMID: 21853194]
• Xiangjun Zheng, Luthur Siu-Lun Cheung, Joyce A. Schroeder, Linan Jiang, Yitshak Zohar A high-performance microsystem for isolating circulating tumor cells Lab on a Chip 2011-10-07 [PMID: 21837324]
• Lee H, Yoon TJ, Figueiredo JL, Swirski FK, Weissleder R Rapid detection and profiling of cancer cells in fine-needle aspirates. Proc. Natl. Acad. Sci. U.S.A., 2009-07-20;106(30):12459-64. 2009-07-20 [PMID: 19620715] (Flow Cytometry, Human)

FAQs for EpCAM/TROP1 Antibody (MAB9601). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM