EpCAM/TROP1 Antibody (158206) [Fluorescein]

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB9601F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 158206
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

EpCAM/TROP1 Antibody (158206) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
  Extracellular domain
• Specificity: Detects human EpCAM/TROP-1 in ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EpCAM/TROP1 Antibody (158206) [Fluorescein]

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca²⁺ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EpCAM/TROP1 Antibody (FAB9601F)(8)

Publications using FAB9601F

• Jang, E;Shin, MK;Kim, H;Lim, JY;Lee, JE;Park, J;Kim, J;Kim, H;Shin, Y;Son, HY;Choi, YY;Hyung, WJ;Noh, SH;Suh, JS;Sung, JY;Huh, YM;Cheong, JH; Clinical molecular subtyping reveals intrinsic mesenchymal reprogramming in gastric cancer cells Experimental & molecular medicine 2023-05-01 [PMID: 37121972] (Flow Cytometry, Human)
• Liu J, Tao M, Zhao W et al. Calcium channel a2 delta 1 is essential for pancreatic tumor-initiating cells through sequential phosphorylation of PKM2 Cellular and molecular gastroenterology and hepatology 10/13/2022 [PMID: 36244646]
• Shibnath Ghatak, Vincent C. Hascall, Nikos Karamanos, Roger R. Markwald, Suniti Misra Interplay Between Chemotherapy-Activated Cancer Associated Fibroblasts and Cancer Initiating Cells Expressing CD44v6 Promotes Colon Cancer Resistance Frontiers in Oncology 2022-08-02 [PMID: 35982950] (Flow Cytometry, Human)
• NH Abu Halim, N Zakaria, K Theva Das, J Lin, MN Lim, KS Fakiruddin, BH Yahaya The Effects of Lentivirus-Mediated Gene Silencing of RAR&beta on the Stemness Capability of Non-Small Cell Lung Cancer Journal of Cancer, 2021-04-19;12(12):3468-3485. 2021-04-19 [PMID: 33995625] (Flow Cytometry, Human)
• HJ Jou, LY Chou, WC Chang, HC Ho, WT Zhang, PY Ling, KH Tsai, SH Chen, TH Chen, PH Lo, M Chen, HT Hsu An Automatic Platform Based on Nanostructured Microfluidic Chip for Isolating and Identification of Circulating Tumor Cells Micromachines, 2021-04-21;12(5):. 2021-04-21 [PMID: 33919456] (ELISA Detection, Human)
• A Jarrar, F Lotti, J DeVecchio, S Ferrandon, G Gantt, A Mace, G Karagkouni, M Orloff, M Venere, M Hitomi, J Lathia, JN Rich, MF Kalady PARP inhibition sensitizes colorectal cancer-initiating cells to chemotherapy Stem Cells, 2018-11-12;0(0):. 2018-11-12 [PMID: 30353615] (Flow Cytometry, Human)
• Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro. BMC Cancer, 2015-05-07;15(0):372. 2015-05-07 [PMID: 25947366] (Flow Cytometry, Human)
• Lotti , Fiorenza, Jarrar , Awad M, Pai , Rish K, Hitomi , Masahiro, Lathia , Justin, Mace , Adam, Gantt , Gerald A, Sukhdeo , Kumar, DeVecchio , Jennifer, Vasanji , Amit, Leahy , Patrick, Hjelmeland , Anita B, Kalady , Matthew, Rich , Jeremy N Chemotherapy activates cancer-associated fibroblasts to maintain colorectal cancer-initiating cells by IL-17A. J Exp Med, 2013-12-09;210(13):2851-72. 2013-12-09 [PMID: 24323355] (Flow Cytometry, Human)

FAQs for EpCAM/TROP1 Antibody (FAB9601F). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM