EPB41L3 Antibody

Images

 
Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in mouse brain lysate
Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in HeLa whole cell lysate
Simple Western: EPB41L3 Antibody [NB110-61027] - Simple Western lane view shows a specific band for EPB41L3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, XpSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml

EPB41L3 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of human EPB41L3 (within residues 400-500). [Swiss-Prot# Q9Y2J2]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPB41L3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 1:50
Application Notes
This EPB41L3 antibody is useful for Western blot, where bands are seen at ~120 and 96 kDa. It detects both isoforms A and B in mouse brain lysates. It detects human isoform A strongly and isoform B more weakly.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Whole Cell Lysate
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB110-61027 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, mouse, rat, primate and Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EPB41L3 Antibody

  • 4.1BFLJ37633
  • DAL1DAL-1,
  • Differentially expressed in adenocarcinoma of the lung protein 1
  • erythrocyte membrane protein band 4.1-like 3
  • KIAA0987band 4.1-like protein 3

Background

Protein 4.1B is a member of a family of similar proteins whose expression is frequently lost in a variety of human tumors, including meningiomas, non-small-cell lung cancers, and breast carcinomas and is frequently down-regulated in human clinical prostate cancer. Studies have shown that Protein 4.1B normally triggers death in metastatic cells, thereby preventing cancer from spreading. A clinical test for Protein 4.1B activity could be useful in assessing the metastatic potential of prostate cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EPB41L3 Antibody (NB110-61027)(2)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(1)
Primate
(1)
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Product General Protocols

View specific protocols for EPB41L3 Antibody (NB110-61027): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EPB41L3 Antibody (NB110-61027). (Showing 1 - 1 of 1 FAQs).

  1. I would want to know: if sample need to be desnaturalized or not, quantity of recombinant protein (in ug) per lane, and recommended concentration of primary and secondary antibody in order to recognise the recombinant protein correctly.
    • We tested NB110-61027 in western blot with reduced and denatured whole mouse brain lysate. Our antibody is recommended to be used at a dilution of 2.0ug/ml for western blot. We perform primary incubation overnight at 4C in blocking buffer (5% nonfat milk in TBST (0.1% tween-20)).

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-61027AF350
Alexa Fluor 405 NB110-61027AF405
Alexa Fluor 488 NB110-61027AF488
Alexa Fluor 532 NB110-61027AF532
Alexa Fluor 594 NB110-61027AF594
Alexa Fluor 647 NB110-61027AF647
Alexa Fluor 700 NB110-61027AF700
Alexa Fluor 750 NB110-61027AF750
Biotin NB110-61027B
DyLight 350 NB110-61027UV
DyLight 405 NB110-61027V
DyLight 488 NB110-61027G
DyLight 550 NB110-61027R
DyLight 594 NB110-61027DL594
DyLight 650 NB110-61027C
DyLight 680 NB110-61027FR
DyLight 755 NB110-61027IR
HRP NB110-61027H
Janelia Fluor 549 NB110-61027JF549
Janelia Fluor 646 NB110-61027JF646

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol EPB41L3