EPB41L3 Antibody - BSA Free

Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in mouse brain lysate

Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in HeLa whole cell lysate

Simple Western: EPB41L3 Antibody [NB110-61027] - Simple Western lane view shows a specific band for EPB41L3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Pm, Xp
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.15 mg/ml

EPB41L3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EPB41L3 Antibody - BSA Free (NB110-61027) is a polyclonal antibody validated for use in WB and Simple Western. Anti-EPB41L3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of human EPB41L3 (within residues 400-500). [Swiss-Prot# Q9Y2J2]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPB41L3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 2 ug/ml
• Application Notes: This EPB41L3 antibody is useful for Western blot, where bands are seen at ~120 and 96 kDa. It detects both isoforms A and B in mouse brain lysates. It detects human isoform A strongly and isoform B more weakly.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 139 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  HeLa Whole Cell Lysate
  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.15 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EPB41L3 Antibody - BSA Free

• 4.1BFLJ37633
• DAL1DAL-1,
• Differentially expressed in adenocarcinoma of the lung protein 1
• erythrocyte membrane protein band 4.1-like 3
• KIAA0987band 4.1-like protein 3

Background

Protein 4.1B is a member of a family of similar proteins whose expression is frequently lost in a variety of human tumors, including meningiomas, non-small-cell lung cancers, and breast carcinomas and is frequently down-regulated in human clinical prostate cancer. Studies have shown that Protein 4.1B normally triggers death in metastatic cells, thereby preventing cancer from spreading. A clinical test for Protein 4.1B activity could be useful in assessing the metastatic potential of prostate cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EPB41L3 Antibody (NB110-61027)(2)

Publications using NB110-61027

• Wong, SY et al. Protein 4.1B suppresses prostate cancer progression and metastasis. Proc Natl Acad Sci U S A. 104(31):12784-9. 2007-07-31 [PMID: 17640904] (WB, Human)
• McCarty, JH et al. An interaction between {alpha}v{beta}8 integrin and Band 4.1B via a highly conserved region of the Band 4.1 C-terminal domain. Proc Natl Acad Sci U S A. 102(38):13479-83. 2005-09-20 [PMID: 16157875] (WB, Primate)

FAQs for EPB41L3 Antibody (NB110-61027). (Showing 1 - 1 of 1 FAQs).

1. I would want to know: if sample need to be desnaturalized or not, quantity of recombinant protein (in ug) per lane, and recommended concentration of primary and secondary antibody in order to recognise the recombinant protein correctly.
   • We tested NB110-61027 in western blot with reduced and denatured whole mouse brain lysate. Our antibody is recommended to be used at a dilution of 2.0ug/ml for western blot. We perform primary incubation overnight at 4C in blocking buffer (5% nonfat milk in TBST (0.1% tween-20)).

Bioinformatics

• Gene Symbol: EPB41L3