Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in mouse brain lysate
Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in HeLa whole cell lysate
Simple Western: EPB41L3 Antibody [NB110-61027] - Simple Western lane view shows a specific band for EPB41L3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
Immunogen affinity purified
This EPB41L3 antibody is useful for Western blot, where bands are seen at ~120 and 96 kDa. It detects both isoforms A and B in mouse brain lysates. It detects human isoform A strongly and isoform B more weakly.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NB110-61027 in the following applications:
Differentially expressed in adenocarcinoma of the lung protein 1
erythrocyte membrane protein band 4.1-like 3
KIAA0987band 4.1-like protein 3
Protein 4.1B is a member of a family of similar proteins whose expression is frequently lost in a variety of human tumors, including meningiomas, non-small-cell lung cancers, and breast carcinomas and is frequently down-regulated in human clinical prostate cancer. Studies have shown that Protein 4.1B normally triggers death in metastatic cells, thereby preventing cancer from spreading. A clinical test for Protein 4.1B activity could be useful in assessing the metastatic potential of prostate cancers.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for EPB41L3 Antibody (NB110-61027). (Showing 1 - 1 of 1 FAQs).
I would want to know: if sample need to be desnaturalized or not, quantity of recombinant protein (in ug) per lane, and recommended concentration of primary and secondary antibody in order to recognise the recombinant protein correctly.
We tested NB110-61027 in western blot with reduced and denatured whole mouse brain lysate. Our antibody is recommended to be used at a dilution of 2.0ug/ml for western blot. We perform primary incubation overnight at 4C in blocking buffer (5% nonfat milk in TBST (0.1% tween-20)).