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EPB4IL2 Antibody

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Immunocytochemistry/Immunofluorescence: EPB4IL2 Antibody [NBP1-06034] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: EPB4IL2 Antibody [NBP1-06034] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: EPB4IL2 Antibody [NBP1-06034] - Staining of paraffin embedded Human Colon tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: EPB4IL2 Antibody [NBP1-06034] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunocytochemistry/ Immunofluorescence: EPB4IL2 Antibody [NBP1-06034] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by ...read more
Immunohistochemistry-Paraffin: EPB4IL2 Antibody [NBP1-06034] - Staining of paraffin embedded Human Uterus tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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EPB4IL2 Antibody Summary

Immunogen
This EPB4IL2 Antibody was developed against a peptide with sequence C-RREVRSPTKAPH corresponding to internal region according to NP_001422.1.
Epitope
C-RREVRSPTKAPH
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPB41L2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
  • Peptide ELISA Detection limit 1:32000
Publications
Read Publication using NBP1-06034.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EPB4IL2 Antibody

  • band 4.1-like protein 2,4.1G
  • DKFZp781D1972
  • DKFZp781H1755,4.1-G
  • erythrocyte membrane protein band 4.1 like-protein 2
  • erythrocyte membrane protein band 4.1-like 2
  • Generally expressed protein 4.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: EPB41L3 Antibody [NB110-61027] - Detection of Band 4.1-like protein 3 in HeLa whole cell lysate
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Western Blot: ERG Antibody [NBP2-60655] - Total protein from Human THP-1, Jurkat and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ERG in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
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Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-52977] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Ezrin Antibody (NBP2-52977) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Flow Cytometry: Nav1.5 Antibody (4G8:1G7) [NB110-61010] - An intracellular stain was performed on U2OS cells with Nav1.5 Antibody [4G8:1G7] NB110-61010PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Immunocytochemistry/Immunofluorescence: Nav1.5 Antibody (4G8-1G7) [NB110-61010] - Figure A: Plasma membrane localisation of Nav1.5 identified by 4G8:1G7 detected by anti-mouse Alexa Fluor-488 conjugated antibody; Figure B, DAP1 co-staining of cell nuclei
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Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IP, RIA, WB
EPB4IL2 Antibody
NBP1-06034
EPB4IL2 Antibody
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA

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Bioinformatics

Gene Symbol EPB41L2
Entrez
Uniprot