EOMES Antibody (644730)

Images

 
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesendoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166) ...read more
EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Monoclonal Antibody (Catalog # MAB6166) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
BG01V human embryonic stem cells differentiated to mesendoderm were stained with Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
644730
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

EOMES Antibody (644730) Summary

Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Specificity
Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EOMES
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publication using
MAB6166 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EOMES Antibody (644730)

  • EOMES
  • eomesodermin (Xenopus laevis) homolog
  • Eomesodermin
  • t box, brain, 2
  • T-box brain protein 2
  • T-box brain2
  • TBR2
  • TBR-2
  • TBR2eomesodermin homolog
  • T-brain-2

Background

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EOMES Antibody (MAB6166)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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FAQs for EOMES Antibody (MAB6166). (Showing 1 - 1 of 1 FAQ).

  1. Please supply a list of eomes antibodies
    • Here is a link to all of our EOMES antibodies

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Bioinformatics

Gene Symbol EOMES
Entrez
Uniprot