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EOMES Antibody (644730)

Images

 
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesendoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166) ...read more
EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Monoclonal Antibody (Catalog # MAB6166) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
BG01V human embryonic stem cells differentiated to mesendoderm were stained with Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
644730
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

EOMES Antibody (644730) Summary

Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Specificity
Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EOMES
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 3.5
using
MAB6166 in the following applications:

Publications
Read Publications using
MAB6166 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EOMES Antibody (644730)

  • EOMES
  • eomesodermin (Xenopus laevis) homolog
  • Eomesodermin
  • t box, brain, 2
  • T-box brain protein 2
  • T-box brain2
  • TBR2
  • TBR-2
  • TBR2eomesodermin homolog
  • T-brain-2

Background

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EOMES Antibody (MAB6166)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.


Filter By Application
ICC
(3)
IHC
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB6166 Applications Species
MS Choe, SJ Kim, ST Oh, CM Bae, WY Choi, KM Baek, JS Kim, MY Lee A simple method to improve the quality and yield of human pluripotent stem cell-derived cerebral organoids Heliyon, 2021;7(6):e07350. 2021 [PMID: 34195446] (ICC, Human) ICC Human
RM Agoglia, D Sun, F Birey, SJ Yoon, Y Miura, K Sabatini, SP Pa?ca, HB Fraser Primate cell fusion disentangles gene regulatory divergence in neurodevelopment Nature, 2021;0(0):. 2021 [PMID: 33731928] (ICC, Human) ICC Human
WS Fagg, N Liu, I Patrikeev, OA Saldarriag, M Motamedi, VL Popov, HL Stevenson, JH Fair Endoderm and Hepatic Progenitor Cells Engraft in the Quiescent Liver Concurrent with Intrinsically Activated Epithelial-to-Mesenchymal Transition Cell Transplantation, 2021;30(0):9636897219937. 2021 [PMID: 33657866] (Western Blot, Human) Western Blot Human
SJ Yoon, LS Elahi, AM Pa?ca, RM Marton, A Gordon, O Revah, Y Miura, EM Walczak, GM Holdgate, HC Fan, JR Huguenard, DH Geschwind, SP Pa?ca Reliability of human cortical organoid generation Nat. Methods, 2019;16(1):75-78. 2019 [PMID: 30573846] (IHC, Human) IHC Human
A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018;7(0):. 2018 [PMID: 30311909] (ICC, Human) ICC Human
Latos P, Sienerth A, Murray A, Senner C, Muto M, Ikawa M, Oxley D, Burge S, Cox B, Hemberger M Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. Genes Dev, 2015;29(23):2435-48. 2015 [PMID: 26584622] (Western Blot, Mouse) Western Blot Mouse

Reviews for EOMES Antibody (MAB6166) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB6166:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen EOMES MAB6166
Enlarge
3
reviewed by:
Verified Customer
IHC-Fr Human 03/30/2020
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedTonsil
SpeciesHuman
LotN/A
Immunohistochemistry-Paraffin EOMES MAB6166
Enlarge
4
reviewed by:
Olivier Etienne
IHC-P Mouse 02/17/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedembryonic mouse brain
SpeciesMouse
Lotcedq0218092

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EOMES Antibody (MAB6166). (Showing 1 - 1 of 1 FAQs).

  1. Please supply a list of eomes antibodies
    • Here is a link to all of our EOMES antibodies

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EOMES Antibody (MAB6166)

Discover related pathways, diseases and genes to EOMES Antibody (MAB6166). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for EOMES Antibody (MAB6166)

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Pathways for EOMES Antibody (MAB6166)

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Blogs on EOMES

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Contact Information

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Calculators

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Verified Customer
03/30/2020
Application: IHC-Fr
Species: Human

Olivier Etienne
02/17/2020
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol EOMES
Uniprot