EOMES Antibody (644730) [Allophycocyanin]

Mesendoderm-differentiated BG01V human embryonic stem cells were stained with Mouse Anti-Human EOMES APC-conjugated Monoclonal Antibody (Catalog # IC6166A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 644730
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

EOMES Antibody (644730) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human EOMES
  Gly471-Pro686
  Accession # O95936
• Specificity: Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EOMES
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EOMES Antibody (644730) [Allophycocyanin]

• EOMES
• eomesodermin (Xenopus laevis) homolog
• Eomesodermin
• t box, brain, 2
• T-box brain protein 2
• T-box brain2
• TBR2
• TBR-2
• TBR2eomesodermin homolog
• T-brain-2

Background

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8⁺ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EOMES Antibody (IC6166A)(3)

Publications using IC6166A

• H Ozawa, H Imazeki, Y Ogiwara, H Kawakubo, K Fukuda, Y Kitagawa, C Kudo-Saito Targeting AURKA in treatment of peritoneal tumor dissemination in gastrointestinal cancer Translational Oncology, 2021-12-10;16(0):101307. 2021-12-10 [PMID: 34902741] (Flow Cytometry, Human)
• S Lunemann, G Martrus, H Goebels, T Kautz, A Langenecke, W Salzberger, M Koch, M J Bunders, B Nashan, KPJM van Gisber, M Altfeld Hobit expression by a subset of human liver-resident CD56(bright) Natural Killer cells Sci Rep, 2017-07-27;7(1):6676. 2017-07-27 [PMID: 28751776] (Flow Cytometry, Human)
• Yijie Geng, Bradley Feng Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis Heliyon 12/5/2015 [PMID: 26705539]

FAQs for EOMES Antibody (IC6166A). (Showing 1 - 1 of 1 FAQs).

1. Please supply a list of eomes antibodies
   • Here is a link to all of our EOMES antibodies

Bioinformatics

• Gene Symbol: EOMES
• Uniprot:
  • O95936(Human)