Human Endostatin ELISA Kit (Colorimetric) Summary
Sensitivity |
10 pg/mL |
Inter-Assay |
CV% < 12% |
Intra-Assay |
CV% < 10% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
COL18A1 |
Applications/Dilutions
Dilutions |
|
Application Notes |
COL18A1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human COL18A1. |
Reactivity Notes
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Assay Diluent A (Item D): Diluent buffer, 0.09% sodium azide as preservative.
- Assay Diluent B (Item E): 5x concentrated buffer.
- Detection Antibody Endostatin (Item F): Biotinylated anti-Human Endostatin(each vial is enough to assay half microplate).
- Endostatin Microplate (Item A): 96 wells coated with anti-Human Endostatin.
- HRP-Streptavidin concentrates (Item G): 400x concentrated HRP-conjugatedstreptavidin.
- Standard Protein (Item C): Human Endostatin. 1 vial is enough to run eachstandard in duplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Endostatin ELISA Kit (Colorimetric)
Background
The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Endostatin ELISA Kit (KA1708). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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