Endostatin Antibody

Western Blot: Endostatin Antibody [NBP1-89587] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Immunohistochemistry: Endostatin Antibody [NBP1-89587] - Staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.

Product Discontinued

Endostatin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENSFM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COL18A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Endostatin Antibody

• antiangiogenic agent
• COL18A1
• collagen alpha-1(XVIII) chain
• collagen, type XVIII, alpha 1
• Endostatin
• FLJ27325
• FLJ34914
• human type XVIII collagen10endostatin
• KNO
• KNO1MGC74745
• Knobloch syndrome, type 1
• KS
• multi-functional protein MFP

Background

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL18A1