Endocan/ESM-1 Antibody [Unconjugated]

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endocan/ESM-1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human Endocan/ESM‑1 (Catalog # 1810‑EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Endocan/ESM‑1 (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Endocan/ESM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1810). The ND50 is typically 1-4 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Endocan/ESM-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Endocan/ESM-1
  Trp20-Arg184
  Accession # Q9NQ30
• Specificity: Detects human Endocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ESM1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1-4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endocan/ESM-1 Antibody [Unconjugated]

• Endocan
• endothelial cell-specific molecule 1
• ESM1
• ESM-1
• IGFBP-rp6

Background

Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF‑ alpha , IL-1 beta , or lipopolysaccharide and down-regulated by IFN‑ gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6).

1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
2. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
3. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217.
4. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
5. Bechard, D. et al. (2001) J. Immunol. 167:3099
6. Scherpereel, A. et al. (2003) Cancer Res. 63:6084.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endocan/ESM-1 Antibody (AF1810)(5)

Publications using AF1810

• KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams Regional specialization and fate specification of bone stromal cells in skeletal development Cell Reports, 2021-07-13;36(2):109352. 2021-07-13 [PMID: 34260921] (IHC, Mouse)
• YC Yang, KF Pan, WJ Lee, JH Chang, P Tan, CC Gu, WM Chang, SF Yang, M Hsiao, KT Hua, MH Chien Circulating proteoglycan endocan mediates EGFR-driven progression of non-small cell lung cancer Cancer Res., 2020-06-19;0(0):. 2020-06-19 [PMID: 32561533] (IHC, Immunoprecipitation, Western Blot, Human)
• Yu P, Chou S, Chen C, Hung H, Lai C, Yang P, Jeng Y, Liaw S, Kuo H, Hsu H, Chen J, Wang W Upregulation of endocan by Epstein-Barr virus latent membrane protein 1 and its clinical significance in nasopharyngeal carcinoma. PLoS ONE, 2013-12-05;8(12):e82254. 2013-12-05 [PMID: 24340011] (ELISA Development, Human)
• Shin JW, Huggenberger R, Detmar M Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis. Blood, 2008-07-09;112(6):2318-26. 2008-07-09 [PMID: 18614759] (Western Blot, Human)
• Rennel E, Mellberg S, Dimberg A, Petersson L, Botling J, Ameur A, Westholm JO, Komorowski J, Lassalle P, Cross MJ, Gerwins P Endocan is a VEGF-A and PI3K regulated gene with increased expression in human renal cancer. Exp. Cell Res., 2007-02-06;313(7):1285-94. 2007-02-06 [PMID: 17362927] (Neutralization, Human)

Bioinformatics

• Gene Symbol: ESM1
• Uniprot:
  • Q9NQ30()