ENC1 Antibody - BSA Free

Immunoprecipitation: ENC1 Antibody - BSA Free [NBP3-46571] - IP Result of anti-ENC1 (IP:, 4ug; Detection: 1:1000) with mouse brain tissue lysate 2640ug.

Western Blot: ENC1 Antibody - BSA Free [NBP3-46571] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (ENC1 antibody) at dilution of 1:400

Immunohistochemistry: ENC1 Antibody - BSA Free [NBP3-46571] - Immunohistochemistry of paraffin-embedded human pancreas using (ENC1 antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ENC1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ENC1 Antibody - BSA Free (NBP3-46571) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ectodermal-neural cortex(with BTB-like domain). (Uniprot# O14682)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 67 kDa, 57 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ENC1 Antibody - BSA Free

• CCL28
• ectodermal-neural cortex 1 (with BTB-like domain)
• ENC-1BTB domain-like (brain)
• FLJ39259
• PIG10KLHL35
• tumor protein p53 inducible protein 10

Background

DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENC1