EMP/MAEA Antibody

Images

 
Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane ...read more
EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

EMP/MAEA Antibody Summary

Immunogen
E. coli-derived recombinant human EMP/MAEA
Met12-Ser66
Accession # Q7L5Y9
Specificity
Detects human, mouse and rat EMP/MAEA in Western blots. Detects recombinant human EMP/MAEA in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
MAEA
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF7288 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMP/MAEA Antibody

  • EMLP
  • EMP
  • EMPCell proliferation-inducing gene 5 protein
  • Erythroblast macrophage protein
  • HLC-10
  • Human lung cancer oncogene 10 protein
  • lung cancer-related protein 10
  • macrophage erythroblast attacher
  • MAEA
  • PIG5
  • proliferation-inducing gene 5

Background

EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-45 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EMP/MAEA Antibody (AF7288)(2)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAEA
Uniprot