EMMPRIN/CD147 Antibody [Biotin]

• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

EMMPRIN/CD147 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
  Thr25-His205
  Accession # Q54A51
• Specificity: Detects human EMMPRIN/CD147 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse EMMPRIN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BSG
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44-66 kDa, variably glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily (1-4). Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) domain and a 41 aa intracellular domain. The ECD contains one C2-type and one V-type Ig-like domain. There is one 385 aa splice variant that contains an extra N-terminal IgCAM domain and is found only in the retina (5). mRNA transcripts, but not protein, have been reported for additional 432, 388, 205, 176, and 174 aa variants.

EMMPRIN is expressed in areas of tissue remodeling, including tumors, endometrium, placenta, skin, and regions undergoing angiogenesis (1, 2, 6-9). It is also expressed in cells with high metabolic activity, such as lymphoblasts, macrophages and tumor cells (2, 10). On cells with elevated metabolic rates, EMMPRIN is often co-expressed with the amino acid transporter CD98h (11). EMMPRIN also interacts with caveolin-1 (via its C2-like domain), and this reduces the level of EMMPRIN glycosylation and subsequent EMMPRIN multimerization and activity (12). EMMPRIN’s TM sequence contains a Glu and a Pro which are important for intracellular interactions with cyclophilins (CyP) (3, 13, 14). CyPA (cyclosporin A receptor) and CyP60 interactions with the TM segment promote leukocyte inflammatory chemotaxis and surface expression of EMMPRIN, respectively (13, 14). An active 22 kDa fragment can be shed from tumor cells by MT1-MMP (1). Tumor cells can also release active, full-length EMMPRIN in microvesicles (15, 16). Functionally, EMMPRIN is known to induce urokinase-type plasminogen activator (uPA), VEGF, hyaluronan, and multiple MMPs (1, 2, 7, 8, 9). Human EMMPRIN (269 aa) shows 58%, 58%, 62%, and 52% aa identity with mouse, rat, bovine, and chicken EMMPRIN, respectively. It also shows 25% and 38% aa identity with the related proteins, embigin and neuroplastin (SDR-1), respectively (4).

1. Gabison, E.E. et al. (2005) Biochimie 87:361.
2. Yurchenko, V. et al. (2006) Immunology 117:301.
3. Kasinrerk, W. et al. (1992) J. Immunol. 149:847.
4. Miyauchi, T. et al. (1991) J. Biochem. 110:770.
5. Hanna, S.M. et al. (2003) BMC Biochem. 4:17.
6. Riethdorf, S. et al. (2006) Int. J. Cancer 119:1800.
7. Braundmeier, A.G. et al. (2006) J. Clin. Endocrinol. Metab. 91:2358.
8. Tang, Y. et al. (2006) Mol. Cancer Res. 4:371.
9. Quemener, C. et al. (2007) Cancer Res. 67:9.
10. Wilson, M.C. et al. (2005) J. Biol. Chem. 280:27213.
11. Xu, D. and M.E. Hemler (2005) Mol. Cell. Proteomics 4:1061.
12. Tang, W. et al. (2004) Mol. Biol. Cell 15:4043.
13. Arora, K. et al. (2005) J. Immunol. 175:517.
14. Pushkarsky, T. et al. (2005) J. Biol. Chem. 280:27866.
15. Egawa, N. et al. (2006) J. Biol. Chem. 281:37576.
16. Sidhu, S.S. et al. (2004) Oncogene 23:956.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG