EML4 Antibody

Images

 
Western Blot: EML4 Antibody [NBP1-86805] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-185
Immunocytochemistry/ Immunofluorescence: EML4 Antibody [NBP1-86805] - Staining of human cell line U-251 MG shows localization to cytosol, intermediate filaments & microtubules. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human lymph node.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex, gallbladder, kidney and lymph node using Anti-EML4 antibody NBP1-86805 (A) shows similar ...read more
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human kidney.
Immunohistochemistry-Paraffin: EML4 Antibody [NBP1-86805] - Staining of human gallbladder.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

EML4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSIIQWKLVEKLSLPQNETVADTTLTKAPVSSTESVIQSNTPTPPPSQPLNETAEEESRISSSPTLLENSLEQTVEPSEDHSEEES
Specificity
Specificity of human EML4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EML4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
IHC reported in scientific literature (PMID: 23490651). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
EML4 Protein (NBP1-86805PEP)
Publications
Read Publications using
NBP1-86805 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25993117).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EML4 Antibody

  • C2orf2
  • echinoderm microtubule associated protein like 4
  • echinoderm microtubule-associated protein-like 4
  • ELP120
  • EMAP-4
  • EMAPL4
  • FLJ10942
  • FLJ32318
  • Restrictedly overexpressed proliferation-associated protein
  • Ropp 120
  • ROPP120DKFZp686P18118

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EML4 Antibody (NBP1-86805)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EML4 Antibody (NBP1-86805) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol EML4
Entrez
Uniprot