EMAP-II/AIMP1 Antibody

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27080] - Analysis of AIMP1 using AIMP1 antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse Raw 4) 3T3 cell lysate probed with 2 ug/ml of AIMP1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: EMAP-II/AIMP1 Antibody [NBP2-27080] - Simple Western lane view shows a specific band for EMAP-II/AIMP1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Po, Bv, ChHa, Eq, Gp, Pm, Ch, Pm, Pm-Or, Rb, RM
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EMAP-II/AIMP1 Antibody Summary

• Immunogen: A portion of amino acids 260-310 from human AIMP1 was used as the immunogen for this antibody.
• Predicted Species: Chimpanzee (100%), Orangutan (100%), Rabbit (94%), Rhesus Macaque (100%), Chicken (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIMP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 2-4ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 48 kDa.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Rhesus Macaque, White-cheeked Gibbon, 89% in New World Monkey, Canine, Opossum, Feline, Frog, Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for EMAP-II/AIMP1 Antibody

• AIMP1
• aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• EMAP-2
• EMAPII
• EMAP-II
• EMAPIImember 1 (endothelialmonocyte-activating)
• endothelial-monocyte activating polypeptide II
• Multisynthase complex auxiliary component p43
• multisynthetase complex auxiliary component p43
• SCYE1

Background

AIMP1, short for Aminoacyl tRNAse synthetase complex iVinteracting multifunctional protein 1, is also called p43 (~43 kDa molecular weight). Along with AIMP2 and AIMP3, AIMP1 is one of the three auxiliary proteins which bind to the aminoacyl tRNase synthetase complex. The AIMPs together with the Aminoacyl tRNAse synthetase, ARS, complex perform a critical role in protein synthesis but also in biological processes such as apoptosis, angiogenesis and inflammation (1, 2). AIMP1 is characterized as a pleiotropic cytokine that enhances TLR1, TLR2, TLR3 and TLR7 expression in BM-DCs. AIMP1 also enhances DC production of IL-6 and IL-12 and increases NF-fUB activity (3, 4). AIMP1 has demonstrated to immune-stimulating activities as well as anti-tumor activities in mouse xenograft models (5). Expression AIMP1 and AIMP2 and 3 proteins are diminished in certain cancers (6). Overall, AIMPs may act as tumor suppressors and perhaps may be considered as a focused target for cancer treatment candidates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIMP1