LAMTOR1 Antibody

Western Blot: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of c11orf59 in human liver protein.

Immunocytochemistry/Immunofluorescence: c11orf59/LAMTOR1 Antibody [NBP1-71689] - C11orf59 antibody was tested at 1:50 in HepG2 cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).

Immunohistochemistry: c11orf59/LAMTOR1 Antibody [NBP1-71689] - Analysis of C11orf59 in mouse pancreas.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

LAMTOR1 Antibody Summary

• Immunogen: A genomic peptide made to a C-terminal region of the human c11orf59/LAMTOR1 protein (within residues 50-161). [Swiss-Prot Q6IAA8]
• Localization: Late endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Cell membrane
• Predicted Species: Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAMTOR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:25-1:100
  • Immunohistochemistry 1:25-1:100
  • Immunohistochemistry-Paraffin 1:25-1:100
  • Western Blot 1:1000
• Application Notes: This c11orf59 antibody is useful for IHC-P, ICC/IF and Western blot, where a band is seen ~22 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Immunogen has 98% identity to bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA, and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for LAMTOR1 Antibody

• c11orf59
• chromosome 11 open reading frame 59
• FLJ20625
• LAMTOR1
• Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1
• late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
• Lipid raft adaptor protein p18
• p18
• p27kip1 releasing factor from RhoA
• p27Kip1-releasing factor from RhoA
• p27RF-RhoC11orf59
• PDRO
• PDROPdro
• Protein associated with DRMs and endosomes
• ragulator complex protein PDRO
• Ragulator1
• RhoA activator C11orf59

Background

c11orf59/LAMTOR1 (ragulator complex protein LAMTOR1 or PDRO), a part of Ragulator complex which contains LAMTOR1-5, is a relatively novel lapidated protein adaptor of the lysosomal membrane which senses and integrates a diverse array of cellular signals, with mTOR kinase activity within the complex being influenced by a variety of nutrients e.g. amino acids, glucose etc., oxygen status, cellular energy levels, and many secreted growth factors such as cytokines and hormones, including insulin. Ragulator is activated by amino acids through a mechanism involving lysosomal V-ATPase, and it functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Ragulator (activated) and Rag GTPases function as a scaffold that recruits mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes and is also required for late endosomes/lysosomes biogenesis. It regulates both the recycling of receptors through endosomes and MAPK signaling pathway through recruitment of some of its components to late endosomes. It implicates in cholesterol homeostasis for regulating LDL uptake and cholesterol release from late endosomes/lysosomes, and may play a role in RHOA activation. LAMTOR1 deregulation is observed in certain pathologies including cancer, diabetes and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for LAMTOR1 Antibody (NBP1-71689) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 03/26/2018

Summary

• Application: Western Blot
• Sample Tested: MEF whole cell lysate
• Species: Mouse
• Lot: A-1

Comments

• Comments: Lysis buffer: 2x Laemmli buffer
  20 µg protein /well
  Ab dilution: 1:1000 in milk-PBST

Bioinformatics

• Gene Symbol: LAMTOR1
• Entrez:
  • 55004(Human)
  • 66508(Mouse)
• Uniprot:
  • Q6IAA8(Human)
  • Q9CQ22(Mouse)