p38 alpha [p Thr180, p Tyr182] Antibody

Images

 
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at ...read more
Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ze, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, Flow-IC, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

p38 alpha [p Thr180, p Tyr182] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH
Modification
p Thr180, p Tyr182
Localization
Cytoplasmic and Nuclear.
Specificity
Specific for the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Predicted Species
Rat (100%), Chicken (100%), Canine (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Flow (Intracellular)
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 24105651).
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB500-138 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 29692779). Mouse reactivity reported in scientific literature (PMID: 24105651).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for p38 alpha [p Thr180, p Tyr182] Antibody

  • MAPK14
  • p38 alpha
  • SAPK2a

Background

P38 is a reactivating kinase that is a member of the MAP kinase family. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. It is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ze, Rt, Bv, Ca, Ch, Pm
Applications: WB, Flow-IC, IHC, IHC-P

Publications for p38 alpha Antibody (NB500-138)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 2 applications: Flow-IC, WB.


Filter By Application
Flow-IC
(1)
WB
(8)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
Rat
(2)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB500-138 Applications Species
Zhao S, Yuan C, Tuo X et al. MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells Chemosphere Aug 15 2020 [PMID: 32828052]
Mukherjee U, Samanta A, Biswas S et al. Bisphenol A-induced oxidative stress, hepatotoxicity and altered estrogen receptor expression in Labeo bata: impact on metabolic homeostasis and inflammatory response Ecotoxicology and Environmental Safety Oct 1 2020 [PMID: 32800225] (WB, Mouse) WB Mouse
Zhang Y, Beketaev I, Segura AM et al. Contribution of Increased Expression of Yin Yang 2 to Development of Cardiomyopathy Front Mol Biosci Mar 3 2020 [PMID: 32195266] (WB, Mouse) WB Mouse
Wu XM, Chen WQ, Hu YW et al. RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways. Front Immunol Apr 10 2018 [PMID: 29692779] (WB, Zebrafish) WB Zebrafish
Brzeszczynska J, Meyer A, McGregor R et al. Alterations in the in vitro and in vivo regulation of muscle regeneration in healthy ageing and the influence of sarcopenia. J Cachexia Sarcopenia Muscle 2017 Dec 06 [PMID: 29214748] (Human) Human
Deobagkar-Lele M, Victor ES, Nandi D. c-Jun NH2 -terminal kinase is a critical node in the death of CD4+ CD8+ thymocytes during Salmonella enterica serovar Typhimurium infection. Eur. J. Immunol. 2014 Jan 1 [PMID: 24105651] (Flow-IC, Mouse)

Details:
Mouse CD4+CD8+ thymocytes, Fig 1A. The results showed that both total and phospho p38MAPk were unaffected by S. typhimurium infection. Antiphospho p38MAPk was obtained from Imgenex.
Flow-IC Mouse
Ito M, Imai M, Muraki M et al. GSTT1 is upregulated by oxidative stress through p38-MK2 signaling pathway in human granulosa cells: possible association with mitochondrial activity. Aging. 2011 Dec 28. [PMID: 22207314] (WB, Human) WB Human
Ito M, Miyado K, Nakagawa K et al. Age-associated changes in the subcellular localization of phosphorylated p38 MAPK in human granulosa cells Mol Hum Reprod 2010 Dec [PMID: 20833869] (WB, Human) WB Human
Ren J Phosvitin Extraction from Egg Yolk and Its Potential as a Functional Food Ingredient for Improving Bone Health Thesis (WB) WB
Show All 12 Publications.

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Bioinformatics

Gene Symbol MAPK14
Entrez
Uniprot