EEF1E1 Antibody

Images

 
Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample(30 ug of whole cell lysate)A:A431 12% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EEF1E1 Antibody [NBP1-32184] - Analysis of paraformaldehyde-fixed HeLa, using EEF1E1 antibody at 1:200 dilution.
Western Blot: EEF1E1 Antibody [NBP1-32184] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

EEF1E1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EEF1E1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF1E1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Rat (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EEF1E1 Antibody

  • AIMP3Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3
  • ARS-interacting multifunctional protein 3
  • Elongation factor p18
  • eukaryotic translation elongation factor 1 epsilon 1
  • eukaryotic translation elongation factor 1 epsilon-1
  • Multisynthase complex auxiliary component p18
  • p18 component of aminoacyl-tRNA synthetase complex
  • P18

Background

This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EEF1E1