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ELF2 Antibody

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Western Blot: ELF2 Antibody [H00001998-B01P] - Analysis of ELF2 expression in transfected 293T cell line by ELF2 polyclonal antibody. Lane 1: ELF2 transfected lysate(63.91 KDa). Lane 2: Non-transfected lysate.
Western Blot: ELF2 Antibody [H00001998-B01P] - Analysis of ELF2 expression in transfected 293T cell line by ELF2 polyclonal antibody. Lane 1: ELF2 transfected lysate(63.91 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ELF2 Antibody [H00001998-B01P] - Analysis of purified antibody to ELF2 on HeLa cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ELF2 Antibody Summary

Immunogen
ELF2 (NP_973728.1, 1 a.a. - 581 a.a.) full-length human protein. MTSAVVDSGGTILELSSNGVENQEESEKVSEYPAVIVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQEVETENVETVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPEFIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGAPLITSTSPTTATSPKVVIQTIPTVMPASTENGDKITMQPAKIITIPATQLAQCQLQTKSNLTGSGSINIVGTPLAVRALTPVSIAHGTPVMRLSMPTQQASGQTPPRVISAVIKGPEVKSEAVAKKQEHDVKTLQLVEEKPADGNKTVTHVVVVSAPSAIALPVTMKTEGLVTCEK
Specificity
ELF2 - E74-like factor 2 (ets domain transcription factor),
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
ELF2
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ELF2 Antibody

  • b
  • E74-like factor 2 (ets domain transcription factor)
  • E74-like factor 2
  • ets family transcription factor ELF2C
  • ETS-related transcription factor Elf-2
  • NERF-1a
  • NERF-1B
  • NERF-2
  • NERFEU32
  • New ETS-related factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ephrin A2 Antibody (3E3) [NBP2-02810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.
Immunocytochemistry/Immunofluorescence: Ephrin A2 Antibody (3E3) [NBP2-02810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
Immunohistochemistry-Paraffin: Ephrin A2 Antibody (3E3) [NBP2-02810] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - IHC analysis of a formalin fixed and paraffin embedded (FFPE) tissue section of mouse brain using 1:100 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.
Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Western blot analysis of GADD153/CHOP expression in HeLa cells treated with 2.5ug/ml tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
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Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.3ug/ml) staining of Human Lymph Node (A), Human Tonsil (B) and Mouse Thymus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: E74 like factor 1 Antibody [NB100-1069] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: E74 like factor 1 Antibody [NB100-1069] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Western Blot: Ets-1 Antibody [NB100-56620] - Analysis of c-ETS-1 in Jurkat cell lysate using 0.5 ug/ml c-Ets-1 antibody.
Species: Hu
Applications: WB
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Western Blot: ETV6/Tel Antibody [NBP1-80695] - Analysis in human cell line HDLM-2.
Immunocytochemistry/Immunofluorescence: ETV6/Tel Antibody [NBP1-80695] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ETV6/Tel Antibody [NBP1-80695] - Staining in human breast and kidney tissues using anti-ETV6 antibody. Corresponding ETV6 RNA-seq data are presented for the same tissues.
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Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in Raw 264.7.
Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in PC-12.
Western Blot: Blk Antibody (7A12) [H00000640-M02] - Analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M02), clone 7A12.Lane 1: BLK transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.
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Western Blot: RUNX1/CBFA2 Antibody [NBP1-89105] - Analysis in control (vector only transfected HEK293T lysate) and rUNX1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody [NBP1-89105] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining in human bone marrow and skeletal muscle tissues using anti-RUNX1 antibody. Corresponding RUNX1 RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: Elf4/MEF Antibody [NBP2-33286] - Staining of human stomach shows high expression.
Immunohistochemistry-Paraffin: Elf4/MEF Antibody [NBP2-33286] - Staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Elf4/MEF Antibody [NBP2-33286] - Staining of human cerebral cortex shows low expression as expected.
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Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6. Lane 1, 293 cells transfected with full-length ATF6.* Lane 2, 293 cells transfected with partial length ATF6 (amino acids 1-373).* Lane 3, Untransfected 293 cells. Western blots were probed with 4 ug/ml of the ATF6 monoclonal antibody and visualized with PicoTect Western Blot Chemiluminescence Substrate (10087K). Film was exposed for 1 min. The top arrow corresponds to the ~90 kDa form of ATF6 described as full-length in the literature. *The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002).
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Image of anti-ATF6. Whole cell protein from Hek293 and HepG2 treated with and without 10 mM DTT was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-ATF6 and detected with an anti-mouse HRP secondary antibody using a maximum sensitivity ECL reagent. Note the loss of the 90 kDa modified ATF6 protein (arrowhead) upon DTT treatment to activate the unfolded protein response.
Western Blot: ATF6 Antibody (70B1413.1) [NBP1-40256] - Analysis of ATF6 in mouse liver tissue using 3 ug/ml of ATF6 antibody and 0.25 ug/ml of GAPDH antibody. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. The ATF6 band may represent under glycosylated or cleaved/active ATF6.
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Western Blot: p97/VCP Antibody (5) [NB120-11433] - Analysis of CA46 cell lysate.
Immunocytochemistry/Immunofluorescence: p97/VCP Antibody (5) [NB120-11433] - Analysis of VCP using VCP Monoclonal antibody (5) shows staining in C6 glioma cells. VCP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing VCP at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06274] - NIH-3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-GRP78/HSPA5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Tie‑2 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Tie‑2 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Tie‑2 at approximately 161 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Tie‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF313) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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MEFV Antibody
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Western Blot: MEFV Antibody [NBP2-17262] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: MEFV Antibody [NBP2-17262] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
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Applications: WB, ICC/IF
Tie-1 Antibody
[Unconjugated]
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Tie‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
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SPDEF Antibody
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Immunocytochemistry/Immunofluorescence: SPDEF Antibody [NBP2-56499] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Species: Hu
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ELF3/ESE-1 Antibody
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Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-ELF3 antibody. Corresponding ELF3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining in human small intestine and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.
Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Applications: WB, IHC, IHC-P
CD79A Antibody
(HM57)
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Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.
Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.
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Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P

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Bioinformatics

Gene Symbol ELF2
Entrez
Uniprot