eIF4G1 Antibody (2A9)

Images

 
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of monoclonal antibody to EIF4G1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in PC-12 (Cat # L012V1).
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in HepG2 (Cat # L019V1).
Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody (M10), clone 2A9. Lane 1: EIF4G1 transfected lysatE (70.95 KDa). Lane 2: ...read more
ELISA: eIF4G1 Antibody (2A9) [H00001981-M10] - Detection limit for recombinant GST tagged EIF4G1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

eIF4G1 Antibody (2A9) Summary

Immunogen
EIF4G1 (NP_886553 1500 a.a. - 1599 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN
Specificity
EIF4G1 (2A9)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EIF4G1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eIF4G1 Antibody (2A9)

  • DKFZp686A1451
  • EIF4F
  • eIF-4G 1
  • eIF4G1
  • EIF-4G1
  • eIF-4-gamma 1
  • EIF4-gamma
  • EIF4GI
  • EIF4Gp220
  • eucaryotic translation initiation factor 4G
  • eukaryotic translation initiation factor 4 gamma 1
  • eukaryotic translation initiation factor 4 gamma, 1
  • P220

Background

The protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF4G1
Entrez
OMIM
Uniprot